FGD6 Protein (AA 1-1430) (Strep Tag)

Catalog No. ABIN3092533

Product Details

Target: FGD6 (FYVE, RhoGEF and PH Domain Containing 6 (FGD6))

Protein Type: Recombinant

Protein Characteristics: AA 1-1430

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FGD6 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MTSAAEIKKP PVAPKPKFVV ANNKPAPPPI APKPDIVISS VPQSTKKMKP AIAPKPKVLK TSPVREIGQS PSRKIMLNLE GHKQELAEST DNFNCKYEGN QSNDYISPMC SCSSECIHKL GHRENLCVKQ LVLEPLEMNE NLENSKIDET LTIKTRSKCD LYGEKAKNQG GVVLKASVLE EELKDALIHQ MPPFISAQKH RPTDSPEMNG GCNSNGQFRI EFADLSPSPS SFEKVPDHHS CHLQLPSDEC EHFETCQDDS EKSNNCFQSS ELEALENGKR STLISSDGVS KKSEVKDLGP LEIHLVPYTP KFPTPKPRKT RTARLLRQKC VDTPSESTEE PGNSDSSSSC LTENSLKINK ISVLHQNVLC KQEQVDKMKL GNKSELNMES NSDAQDLVNS QKAMCNETTS FEKMAPSFDK DSNLSSDSTT VDGSSMSLAV DEGTGFIRCT VSMSLPKQLK LTCNEHLQSG RNLGVSAPQM QKESVIKEEN SLRIVPKKPQ RHSLPATGVL KKAASEELLE KSSYPSSEEK SSEKSLERNH LQHLCAQNRG VSSSFDMPKR ASEKPVWKLP HPILPFSGNP EFLKSVTVSS NSEPSTALTK PRAKSLSAMD VEKCTKPCKD STKKNSFKKL LSMKLSICFM KSDFQKFWSK SSQLGDTTTG HLSSGEQKGI ESDWQGLLVG EEKRSKPIKA YSTENYSLES QKKRKKSRGQ TSAANGLRAE SLDDQMLSRE SSSQAPYKSV TSLCAPEYEN IRHYEEIPEY ENLPFIMAIR KTQELEWQNS SSMEDADANV YEVEEPYEAP DGQLQLGPRH QHSSSGASQE EQNDLGLGDL PSDEEEIINS SDEDDVSSES SKGEPDPLED KQDEDNGMKS KVHHIAKEIM SSEKVFVDVL KLLHIDFRDA VAHASRQLGK PVIEDRILNQ ILYYLPQLYE LNRDLLKELE ERMLHWTEQQ RIADIFVKKG PYLKMYSTYI KEFDKNIALL DEQCKKNPGF AAVVREFEMS PRCANLALKH YLLKPVQRIP QYRLLLTDYL KNLIEDAGDY RDTQDALAVV IEVANHANDT MKQGDNFQKL MQIQYSLNGH HEIVQPGRVF LKEGILMKLS RKVMQPRMFF LFNDALLYTT PVQSGMYKLN NMLSLAGMKV RKPTQEAYQN ELKIESVERS FILSASSATE RDEWLEAISR AIEEYAKKRI TFCPSRSLDE ADSENKEEVS PLGSKAPIWI PDTRATMCMI CTSEFTLTWR RHHCRACGKI VCQACSSNKY GLDYLKNQPA RVCEHCFQEL QKLDHQHSPR IGSPGNHKSP SSALSSVLHS IPSGRKQKKI PAALKEVSAN TEDSSMSGYL YRSKGNKKPW KHFWFVIKNK VLYTYAASED VAALESQPLL GFTVIQVKDE NSESKVFQLL HKNMLFYVFK AEDAHSAQKW IEAFQEGTIL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FGD6 (FYVE, RhoGEF and PH Domain Containing 6 (FGD6))

Alternative Name: FGD6 (FGD6 Products)

Synonyms: ZFYVE24 Protein, AA123052 Protein, Etohd4 Protein, FYVE, RhoGEF and PH domain containing 6 Protein, FGD6 Protein, Fgd6 Protein

Background: FYVE, RhoGEF and PH domain-containing protein 6 (Zinc finger FYVE domain-containing protein 24),FUNCTION: May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. May play a role in regulating the actin cytoskeleton and cell shape (By similarity). {ECO:0000250}.

Molecular Weight: 160.8 kDa

UniProt: Q6ZV73