HTRA3 Protein (AA 18-453) (His tag)

Catalog No. ABIN3092994

Product Details

Target: HTRA3 (HtrA Serine Peptidase 3 (HTRA3))

Protein Type: Recombinant

Protein Characteristics: AA 18-453

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This HTRA3 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: MREPPAAPCP ARCDVSRCPS PRCPGGYVPD LCNCCLVCAA SEGEPCGGPL DSPCGESLEC VRGLCRCRWS HAVCGTDGHT YANVCALQAA SRRALQLSGT PVRQLQKGAC PLGLHQLSSP RYKFNFIADV VEKIAPAVVH IELFLRHPLF GRNVPLSSGS GFIMSEAGLI ITNAHVVSSN SAAPGRQQLK VQLQNGDSYE ATIKDIDKKS DIATIKIHPK KKLPVLLLGH SADLRPGEFV VAIGSPFALQ NTVTTGIVST AQREGRELGL RDSDMDYIQT DAIINYGNSG GPLVNLDGEV IGINTLKVTA GISFAIPSDR ITRFLTEFQD KQIKDWKKRF IGIRMRTITP SLVDELKASN PDFPEVSSGI YVQEVAPNSP SQRGGIQDGD IIVKVNGRPL VDSSELQEAV LTESPLLLEV RRGNDDLLFS IAPEVVMHHHHHH
Sequence including C-terminal His-tag.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human HTRA3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).
• Tag Location: C-terminal His Tag

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: HTRA3 (HtrA Serine Peptidase 3 (HTRA3))

Alternative Name: HTRA3 (HTRA3 Products)

Synonyms: HTRA3 Protein, Prsp Protein, Tasp Protein, 2210021K23Rik Protein, 9530081K03Rik Protein, RGD1308120 Protein, Trypsin-like serine protease Protein, HtrA serine peptidase 3 Protein, htrA3 Protein, HTRA3 Protein, Htra3 Protein

Background: Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed:21321049, PubMed:22229724). {ECO:0000250|UniProtKB:Q9D236, ECO:0000269|PubMed:21321049, ECO:0000269|PubMed:22229724}.

Molecular Weight: 47.9 kDa Including tag.

UniProt: P83110