MACF1 Protein (AA 1-1291) (His tag)

Catalog No. ABIN3093175

Product Details

Target: MACF1 (Microtubule-Actin Crosslinking Factor 1 (MACF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1291

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This MACF1 protein is labelled with His tag.

Application: Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS)

Sequence: MPPNFPEFAE RIEASLSEVS EAGASNPSLQ EKKESSSALT ESSGHLDHRE PQSESVTLEH VSKSIGIPEV QDFKNLSGDC QDFRFQQHSA NPPHEFQPVE SEAVATSGNT DVMQESRFSS ATWPRATKSL AKGGFSEKQH PLGDTACTVE MPPLSPCLSE ELLDPELHVL ITPSLREKTE SELKFEEDER WIMMEAEGEW EEEKLSDREK TFLMADEKNS LADIFEEREQ ANTAVVEDGS DCLAAVLRTF GHLSLGQICC PDDPQPAKDQ LATVPKDIPL DCDCVLTGED ILGEVANRTA QGLEGLVSDS ACTVGTIDAE QLSDTDSVQM FLELEKECLC EEGVTPLVEL QNQISSEGLA ASQDAENLLV ISHFSGAALE KEQHLGLLHV RAKDYDTRLD CGYFNTLDSS QVPNAVELIA HVDIMRDTST VSKEECEKVP FSPRTAEFKS RQPADLDSLE KLDPGGLLNS DHRVSHEEKL SGFIASELAK DNGSLSQGDC SQTEGNGEEC IERVTFSFAF NHELTDVTSG PEVEVLYESN LLTDEIHLES GNVTVNQENN SLTSMGNVVT CELSVEKVCD EDGEAKELDY QATLLEDQAP AHFHRNFPEQ VFQDLQRKSP ESEILSLHLL VEELRLNPDG VETVNDTKPE LNVASSEGGE MERRDSDSFL NIFPEKQVTK AGNTEPVLEE WIPVLQRPSR TAAVPTVKDA LDAALPSPEE GTSIAAVPAP EGTAVVAALV PFPHEDILVA SIVSLEEEDV TAAAVSAPER ATVPAVTVSV PEGTAAVAAV SSPEETAPAV AAAITQEGMS AVAGFSPEWA ALAITVPITE EDGTPEGPVT PATTVHAPEE PDTAAVRVST PEEPASPAAA VPTPEEPTSP AAAVPTPEEP TSPAAAVPPP EEPTSPAAAV PTPEEPTSPA AAVPTPEEPT SPAAAVPTPE EPTSPAAAVP TPEEPTSPAA AVPTPEEPTS PAAAVPTPEE PASPAAAVPT PEEPASPAAA VPTPEEPAFP APAVPTPEES ASAAVAVPTP EESASPAAAV PTPAESASFA AVVATLEEPT SPAASVPTPA AMVATLEEFT SPAASVPTSE EPASLAAAVS NPEEPTSPAA AVPTLEEPTS SAAAVLTPEE LSSPAASVPT PEEPASPAAA VSNLEEPASP AAAVPTPEVA AIPAASVPTP EVPAIPAAAV PPMEEVSPIG VPFLGVSAHT DSVPISEEGT PVLEEASSTG MWIKEDLDSL VFGIKEVTST VLHGKVPLAA TAGLNSDEVI VHFDSGKGLK SKVRFAGLTW W
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human KIAA0754 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MACF1 (Microtubule-Actin Crosslinking Factor 1 (MACF1))

Alternative Name: KIAA0754 (MACF1 Products)

Synonyms: ABP620 Protein, ACF7 Protein, MACF Protein, OFC4 Protein, Acf7 Protein, Aclp7 Protein, R74989 Protein, mKIAA0465 Protein, MACF1 Protein, fc46g01 Protein, wu:fc46g01 Protein, si:rp71-45k5.7 Protein, acf7 Protein, macf Protein, ofc4 Protein, abp620 Protein, DKFZp469K2016 Protein, Kiaa0754 Protein, mKIAA0754 Protein, microtubule-actin crosslinking factor 1 Protein, microtubule-actin crosslinking factor 1a Protein, microtubule-actin cross-linking factor 1 Protein, dual specificity protein kinase CLK3 Protein, microtubule-actin cross-linking factor 1, isoforms 1/2/3/5 Protein, KIAA0754 Protein, RIKEN cDNA D830031N03 gene Protein, MACF1 Protein, Macf1 Protein, macf1a Protein, macf1 Protein, LOC100175356 Protein, LOC100514848 Protein, LOC100550622 Protein, KIAA0754 Protein, D830031N03Rik Protein

Molecular Weight: 136.1 kDa Including tag.

UniProt: O94854