LY9 Protein (AA 48-655) (rho-1D4 tag)

Catalog No. ABIN3093592

Product Details

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Protein Type: Recombinant

Protein Characteristics: AA 48-655

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This LY9 protein is labelled with rho-1D4 tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: KDSAPTVVSG ILGGSVTLPL NISVDTEIEN VIWIGPKNAL AFARPKENVT IMVKSYLGRL DITKWSYSLC ISNLTLNDAG SYKAQINQRN FEVTTEEEFT LFVYEQLQEP QVTMKSVKVS ENFSCNITLM CSVKGAEKSV LYSWTPREPH ASESNGGSIL TVSRTPCDPD LPYICTAQNP VSQRSSLPVH VGQFCTDPGA SRGGTTGETV VGVLGEPVTL PLALPACRDT EKVVWLFNTS IISKEREEAA TADPLIKSRD PYKNRVWVSS QDCSLKISQL KIEDAGPYHA YVCSEASSVT SMTHVTLLIY RRLRKPKITW SLRHSEDGIC RISLTCSVED GGNTVMYTWT PLQKEAVVSQ GESHLNVSWR SSENHPNLTC TASNPVSRSS HQFLSENICS GPERNTKLWI GLFLMVCLLC VGIFSWCIWK RKGRCSVPAF CSSQAEAPAD TPEPTAGHTL YSVLSQGYEK LDTPLRPARQ QPTPTSDSSS DSNLTTEEDE DRPEVHKPIS GRYEVFDQVT QEGAGHDPAP EGQADYDPVT PYVTEVESVV GENTMYAQVF NLQGKTPVSQ KEESSATIYC SIRKPQVVPP PQQNDLEIPE SPTYENFT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human LY9 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Alternative Name: LY9 (CD229 Products)

Synonyms: LY9 Protein, CD229 Protein, SLAMF3 Protein, hly9 Protein, mLY9 Protein, AI893573 Protein, Lgp100 Protein, Ly-9 Protein, T100 Protein, lymphocyte antigen 9 Protein, LY9 Protein, Ly9 Protein

Background: Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion, the costimulatory activity requires SH2D1A (PubMed:22184727). Promotes recruitment of RORC to the IL-17 promoter (PubMed:22989874). May be involved in the maintenance of peripheral cell tolerance by serving as a negative regulator of the immune response. May disable autoantibody responses and inhibit IFN-gamma secretion by CD4(+) T-cells. May negatively regulate the size of thymic innate CD8(+) T-cells and the development of invariant natural killer T (iNKT) cells (By similarity). {ECO:0000250|UniProtKB:Q01965, ECO:0000269|PubMed:22184727, ECO:0000269|PubMed:22989874}.

Molecular Weight: 68.2 kDa Including tag.

UniProt: Q9HBG7

Pathways: BCR Signaling