Lamin A/C Protein (LMNA) (AA 1-646) (His tag)

Catalog No. ABIN3093600

Product Details

Target: Lamin A/C (LMNA)

Protein Type: Recombinant

Protein Characteristics: AA 1-646

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Lamin A/C protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: METPSQRRAT RSGAQASSTP LSPTRITRLQ EKEDLQELND RLAVYIDRVR SLETENAGLR LRITESEEVV SREVSGIKAA YEAELGDARK TLDSVAKERA RLQLELSKVR EEFKELKARN TKKEGDLIAA QARLKDLEAL LNSKEAALST ALSEKRTLEG ELHDLRGQVA KLEAALGEAK KQLQDEMLRR VDAENRLQTM KEELDFQKNI YSEELRETKR RHETRLVEID NGKQREFESR LADALQELRA QHEDQVEQYK KELEKTYSAK LDNARQSAER NSNLVGAAHE ELQQSRIRID SLSAQLSQLQ KQLAAKEAKL RDLEDSLARE RDTSRRLLAE KEREMAEMRA RMQQQLDEYQ ELLDIKLALD MEIHAYRKLL EGEEERLRLS PSPTSQRSRG RASSHSSQTQ GGGSVTKKRK LESTESRSSF SQHARTSGRV AVEEVDEEGK FVRLRNKSNE DQSMGNWQIK RQNGDDPLLT YRFPPKFTLK AGQVVTIWAA GAGATHSPPT DLVWKAQNTW GCGNSLRTAL INSTGEEVAM RKLVRSVTVV EDDEDEDGDD LLHHHHGSHC SSSGDPAEYN LRSRTVLCGT CGQPADKASA SGSGAQVGGP ISSGSSASSV TVTRSYRSVG GSGGGSFGDN LVTRSY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human LMNA Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Lamin A/C (LMNA)

Alternative Name: LMNA (LMNA Products)

Synonyms: CDCD1 Protein, CDDC Protein, CMD1A Protein, CMT2B1 Protein, EMD2 Protein, FPL Protein, FPLD Protein, FPLD2 Protein, HGPS Protein, IDC Protein, LDP1 Protein, LFP Protein, LGMD1B Protein, LMN1 Protein, LMNC Protein, LMNL1 Protein, PRO1 Protein, Dhe Protein, 2459 Protein, 74/76 Protein, CG6944 Protein, D5 Protein, DM[[O]] Protein, Dm Protein, Dm(0) Protein, Dm0 Protein, Dm1 Protein, Dm2 Protein, DmLamin Protein, DmO Protein, Dm[[0]] Protein, Dm[[1]] Protein, Dm[[2]] Protein, Dm[[mit]] Protein, Dm[[o]] Protein, Dmel\\CG6944 Protein, Dmo Protein, LAM Protein, Lam Dm[[0]] Protein, Lam(Dm0) Protein, LamDm[[0]] Protein, LamDm[[o]] Protein, Lam[[Dm0]] Protein, Lamin Dm[[0]] Protein, jf27 Protein, l(2)04643 Protein, l(2)25Ec Protein, l(2)gdh-7 Protein, l(2)gdh7 Protein, l(2)jf27 Protein, lam Protein, lamDm0 Protein, lamDm[[0]] Protein, lamin Protein, lamin Dm0 Protein, lamin Dm[[0]] Protein, misg Protein, nlam Protein, cb948 Protein, fk66d12 Protein, wu:fk66d12 Protein, lmna-A Protein, fpl Protein, idc Protein, lfp Protein, cddc Protein, emd2 Protein, fpld Protein, hgps Protein, ldp1 Protein, lmn1 Protein, lmnc Protein, pro1 Protein, cdcd1 Protein, cmd1a Protein, cmt2b1 Protein, lgmd1b Protein, Lamin-A Protein, lamin-L(III)-like Protein, lamin A/C Protein, lamin A Protein, Lamin Protein, lamin A/C L homeolog Protein, lamin-L(III)-like Protein, LMNA Protein, Lmna Protein, Lam Protein, lmna Protein, lmna.L Protein, LMINA Protein

Background: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone., Prelamin-A/C can accelerate smooth muscle cell senescence. It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence.

Molecular Weight: 73.2 kDa Including tag.

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus