RFX7 Protein (AA 1-1363) (His tag)
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- Target See all RFX7 products
- RFX7 (Regulatory Factor X 7 (RFX7))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1363
- Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This RFX7 protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MSSSRAQQMH AFSWIRNTLE EHPETSLPKQ EVYDEYKSYC DNLGYHPLSA ADFGKIMKNV FPNMKARRLG TRGKSKYCYS GLRKKAFVHM PTLPNLDFHK TGDGLEGAEP SGQLQNIDEE VISSACRLVC EWAQKVLSQP FDTVLELARF LVKSHYIGTK SMAALTVMAA APAGMKGITQ PSAFIPTAES NSFQPQVKTL PSPIDAKQQL QRKIQKKQQE QKLQSPLPGE SAAKKSESAT SNGVTNLPNG NPSILSPQPI GIVVAAVPSP IPVQRTRQLV TSPSPMSSSD GKVLPLNVQV VTQHMQSVKQ APKTPQNVPA SPGGDRSARH RYPQILPKPA NTSALTIRSP TTVLFTSSPI KTAVVPASHM SSLNVVKMTT ISLTPSNSNT PLKHSASVSS ATGTTEESRS VPQIKNGSVV SLQSPGSRSS SAGGTSAVEV KVEPETSSDE HPVQCQENSD EAKAPQTPSA LLGQKSNTDG ALQKPSNEGV IEIKATKVCD QRTKCKSRCN EMLPGTSTGN NQSTITLSVA SQNLTFTSSS SPPNGDSINK DPKLCTKSPR KRLSSTLQET QVPPVKKPIV EQLSAATIEG QKQGSVKKDQ KVPHSGKTEG STAGAQIPSK VSVNVSSHIG ANQPLNSSAL VISDSALEQQ TTPSSSPDIK VKLEGSVFLL DSDSKSVGSF NPNGWQQITK DSEFISASCE QQQDISVMTI PEHSDINDLE KSVWELEGMP QDTYSQQLHS QIQESSLNQI QAHSSDQLPL QSELKEFEPS VSQTNESYFP FDDELTQDSI VEELVLMEQQ MSMNNSHSYG NCLGMTLQSQ SVTPGAPMSS HTSSTHFYHP IHSNGTPIHT PTPTPTPTPT PTPTPTPTSE MIAGSQSLSR ESPCSRLAQT TPVDSALGSS RHTPIGTPHS NCSSSVPPSP VECRNPFAFT PISSSMAYHD ASIVSSSPVK PMQRPMATHP DKTKLEWMNN GYSGVGNSSV SGHGILPSYQ ELVEDRFRKP HAFAVPGQSY QSQSRHHDTH FGRLTPVSPV QHQGATVNNT NKQEGFAVPA PLDNKGTNSS ASSNFRCRSV SPAVHRQRNL SGSTLYPVSN IPRSNVTPFG SPVTPEVHVF TNVHTDACAN NIAQRSQSVP LTVMMQTAFP NALQKQANSK KITNVLLSKL DSDNDDAVRG LGMNNLPSNY TARMNLTQIL EPSTVFPSAN PQNMIDSSTS VYEFQTPSYL TKSNSTGQIN FSPGDNQAQS EIGEQQLDFN STVKDLLSGD SLQTNQQLVG QGASDLTNTA SDFSSDIRLS SELSGSINDL NTLDPNLLFD PGRQQGQDDE ATLEELKNDP LFQQICSESM NSMTSSGFEW IESKDHPTVE MLG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human RFX7 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- RFX7 (Regulatory Factor X 7 (RFX7))
- Alternative Name
- RFX7 (RFX7 Products)
- Synonyms
- RFXDC2 Protein, 2510005N23Rik Protein, 9930116O05Rik Protein, D130086K05Rik Protein, Rfxdc2 Protein, regulatory factor X7 Protein, regulatory factor X, 7 Protein, RFX7 Protein, Rfx7 Protein
- Molecular Weight
- 147.8 kDa Including tag.
- UniProt
- Q2KHR2
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