REXO1 Protein (AA 1-1221) (Strep Tag)

Catalog No. ABIN3095002

Product Details

Target: REXO1 (REX1, RNA Exonuclease 1 Homolog (REXO1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1221

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This REXO1 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Sequence: MLRSTGFFRA IDCPYWSGAP GGPCRRPYCH FRHRGARGSG APGDGGEAPP AAGLGYDPYN PELPKPPAQR ENGTLGLGEE PRPDVLELEL VNQAIEAVRS EVELEQRRYR ELLETTREHR SAEAPALAPR GPNASPTVGP DEDAFPLAFD YSPGSHGLLS PDAGYQPTPL AAPAEPGSKY SLASLDRGQG RGGGGGGALE YVPKAVSQPR RHSRPVPSGK YVVDNSRPPT DLEYDPLSNY SARHLSRASS RDERAAKRPR GSRGSEPYTP APKKLCDPFG SCDARFSDSE DEAATVPGNE PTTASTPKAR ADPEIKATGQ PPSKEGLEAE GGGLRETKET AVQCDVGDLQ PPPAKPASPA QVQSSQDGGC PKEGKPKKKK TGAPPAPSCK DGAQGKDKTK DKGRGRPVEK PRADKKGPQA SSPRRKAERP EGTKKKPSSA TPVATSGKGR PDRPARRPSP TSGDSRPAAG RGPPRPLQLP DRKSTKAPSG KLVERKARSL DEGASQDAPK LKKRALSHAD LFGDESEDEA AGPGVPSVWP SALPSLSSDS DSDSDSSLGF PEAQGPPKRL KASPPPSPAP SSSSSSSSST SSAGADVDYS ALEKEVDFDS DPMEECLRIF NESTSVKTED RGRLARQPPK EEKSEEKGLS GLTTLFPGQK RRISHLSKQG QEVEPPRRGP AVPPARPPTA QEVCYLRAQQ AQRASASLLQ APARLAEKSP SVHISAPGEK RRIAHIPNPR LAAAPTGAKR TLAASGSQSS NGPEPGGQQL KTRTLSGMAS KTTTTIIPKR IAHSPSLQSL KKPIIPKEFG GKVPTVIRQR YLNLFIEECL KFCTSNQEAI EKALNEEKVA YDRSPSKNIY LNVAVNTLKK LRGLAPSAVP GLSKTSGRRV VSHEVVLGGR LAAKTSFSLS RPSSPRVEDL KGAALYSRLR EYLLTQDQLK ENGYPFPHPE RPGGAIIFTA EEKRPKDSSC RTCCRCGTEY LVSSSGRCIR DEECYYHWGR LRRNRVAGGW ETQYMCCSAA AGSVGCQVAK QHVQDGRKER LEGFVKTFEK ELSGDTHPGI YALDCEMSYT TYGLELTRVT VVDTDVHVVY DTFVKPDNEI VDYNTRFSGV TEADLADTSV TLRDVQAVLL SMFSADTILI GHSLESDLLA LKVIHSTVVD TSVLFPHRLG LPYKRSLRNL MADYLRQIIQ DNVDGHSSSE DAGACMHLVI WKVREDAKTK R
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: REXO1 (REX1, RNA Exonuclease 1 Homolog (REXO1))

Alternative Name: REXO1 (REXO1 Products)

Synonyms: ELOABP1 Protein, EloA-BP1 Protein, REX1 Protein, TCEB3BP1 Protein, 1700021P10Rik Protein, 2610511M11Rik Protein, AW050347 Protein, Tceb3bp1 Protein, fb77b11 Protein, wu:fb77b11 Protein, si:dkey-45m5.5 Protein, rex1 Protein, eloabp1 Protein, eloa-bp1 Protein, tceb3bp1 Protein, RNA exonuclease 1 homolog Protein, REX1, RNA exonuclease 1 Protein, REX1, RNA exonuclease 1 homolog Protein, REXO1 Protein, Rexo1 Protein, rexo1 Protein

Background: RNA exonuclease 1 homolog (EC 3.1.-.-) (Elongin-A-binding protein 1) (EloA-BP1) (Transcription elongation factor B polypeptide 3-binding protein 1),FUNCTION: Seems to have no detectable effect on transcription elongation in vitro. {ECO:0000269|PubMed:12943681}.

Molecular Weight: 131.5 kDa

UniProt: Q8N1G1