RBM16 Protein (AA 1-1271) (Strep Tag)

Catalog No. ABIN3095233

Product Details

Target: RBM16 (RNA Binding Motif Protein 16 (RBM16))

Protein Type: Recombinant

Protein Characteristics: AA 1-1271

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This RBM16 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MEAVKTFNSE LYSLNDYKPP ISKAKMTQIT KAAIKAIKFY KHVVQSVEKF IQKCKPEYKV PGLYVIDSIV RQSRHQFGQE KDVFAPRFSN NIISTFQNLY RCPGDDKSKI VRVLNLWQKN NVFKSEIIQP LLDMAAGIPP PVVTPVLAST TTAMSNTPGT PVTPVTPANV VQGLPDPWVS QITNTDTLAA VAQILQSPQG QQLQQLIQTL QIQQQKPQPS ILQALDAGLV VQLQALTAQL TAAAAAANTL TPLEQGVSFN KKLMDRFDFG EDSEHSEEPK KEIPASQLSH VSESVNNSIF HQIAEQLQQQ NLEHLRQQLL EQQQPQKATP QDSQEGTFGS EHSASPSQGS SQQHFLEPEV NLDDSIDIQQ QDMDIDEGQD GVEEEVFEQE AKKVAVRSRS RTHSRSRSRS PRKRRSRSRS GSRKRKHRKR SRSRSRERKR KSSRSYSSER RAREREKERQ KKGLPPIRSK TLSVCSTTLW VGQVDKKATQ QDLTNLFEEF GQIESINMIP PRGCAYVCMV HRQDAFRALQ KLSSGSYKIG SKVIKIAWAL NKGVKTEYKQ FWDVDLGVTY IPWEKVKVDD LEGFAEGGMI DQETVNTEWE TVKSSEPVKE TVQTTQSPTP VEKETVVTTQ AEVFPPPVAM LQIPVAPAVP TVSLVPPAFP VSMPVPPPGF SPIPPPPFLR ASFNPSQPPP GFMPPPVPPP VVPPPTIPPV VPTSLVQPSL SMTPETVKDV GFGSLVIPGG SVASNLATSA LPAGNVFNAP TKQAEPEEKV PHLIDHQISS GENTRSVIPN DISSNAAILG GQPPNVTSNS GILGVQRPNV SSNSEILGVR PSNVSSSSGI IAAQPPNILN NSGILGIQPP SVSNSSGLLG VLPPNIPNNS GLVGVQPPNV PNTPGLLGTQ PPAGPQNLPP LSIPNQRMPT MPMLDIRPGL IPQAPGPRFP LIQPGIPPQR GIPPPSVLDS ALHPPPRGPF PPGDIFSQPE RPFLAPGRQS VDNVTNPEKR IPLGNDNIQQ EGDRDYRFPP IETRESISRP PPVDVRDVVG RPIDPREGPG RPPLDGRDHF GRPPVDIREN LVRPGIDHLG RRDHFGFNPE KPWGHRGDFD EREHRVLPVY GGPKGLHEER GRFRSGNYRF DPRSGPWNRG FGQEVHRDFD DRRRPWERQR DRDDRDFDFC REMNGNRLGR DRIQNTWVPP PHARVFDYFE GATSQRKGDN VPQVNGENTE RHAQPPPIPV QNDPELYEKL TSSNEINKEK SDTVADIESE PVVESTETEG T
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RBM16 (RNA Binding Motif Protein 16 (RBM16))

Alternative Name: SCAF8 (RBM16 Products)

Synonyms: RBM16 Protein, A630086M08Rik Protein, A930036P18Rik Protein, AA408306 Protein, AI447644 Protein, AI448652 Protein, AU015035 Protein, Rbm16 Protein, mKIAA1116 Protein, MGC84961 Protein, fd99c08 Protein, wu:fc50c06 Protein, wu:fd99c08 Protein, SR-related CTD associated factor 8 Protein, SR-related CTD-associated factor 8 Protein, SR-related CTD associated factor 8 L homeolog Protein, SCAF8 Protein, Scaf8 Protein, scaf8.L Protein, scaf8 Protein

Background: SR-related and CTD-associated factor 8 (CDC5L complex-associated protein 7) (RNA-binding motif protein 16),FUNCTION: Anti-terminator protein required to prevent early mRNA termination during transcription (PubMed:31104839). Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins (PubMed:31104839). Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation (PubMed:31104839). Independently of SCAF4, also acts as a positive regulator of transcript elongation (PubMed:31104839). {ECO:0000269|PubMed:31104839}.

Molecular Weight: 140.5 kDa

UniProt: Q9UPN6