SCAND3 Protein (AA 1-1325) (Strep Tag)
Crystallography grade
SCAND3
Origin: Human
Host: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA
-
- Target See all SCAND3 products
- SCAND3 (SCAN Domain Containing 3 (SCAND3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1325
- Origin
- Human
-
Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This SCAND3 protein is labelled with Strep Tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- Sequence
-
MEAVSRVFPA LAGQAPEEQG EIIKVKVKEE DHTWDQESAL RRNLSYTREL SRQRFRQFCY QETPGPREAL SQLRELCRQW LNPEIHTKEQ ILELLVLEQF LTILPEELQS WVREHNPESG EEVVTLLEDL ERELDEPRQQ VSQGTYGQEV SMEEMIPLDS AKESLGTQLQ SMEDRMECES PEPHPLQDNG SFLWFSMMSQ SMGGDNLSSL DTNEAEIEPE NMREKFFRSL ARLLENKSNN TKIFSKAKYC QLIKEVKEAK AKAKKESVDY RRLARFDVIL VQGNEKLIEA VNGETDKIRY YLHSEDLFDI LHNTHLSIGH GGRTRMEKEL QAKYKNITKE VIMLYLTLCK PCQQKNSKLK KVLTSKSIKE VSSRCQVDLI DMQLNPDGEY RFILHYQDLC TKLTFLRSLK SKRPTEVAHA LLDIFTIIGA PSVLQSDNGR EFSSQVVSEL SNIWPELKIV HGKSQTCQSQ SSAEQTEDIR KRIFSWMQTN NSSHWTEFLW FIQMSQNQPY HRSMQQTPCE SAFSSEAKLG LSHSQLTEEL VASLHTENEL DQADKELENT LRAQYEENIE TGTDSSDIEE NLSVTPKVAE KSPPESRLRF LSCVVCEKEC TGVNSCISCD GNIHAICGVP SQHGTEGCGR QITCSLCYET STMKRKHDEI QRSLPVKPSK MLKPSGTPFS PDKVGDWMAK QASLDFFVKK RHAFSEHSSS NKRNVNNRSY PEEGKTKRVH ASFTRKYDPS YIEFGFVAVI DGEVLKPQCI ICGDVLANEA MKPSKLKRHL YSKHKEISSQ PKEFFERKSS ELKSQPKQVF NVSHINISAL RASYKVALPV AKSKTPYTIA ETLVKDCIKE VCLEMLGESA AKKVAQVPLS NDTIARRIQE LANDMEDQLI EQIKLAKYFS LQLDECRDIA NMIILLVYVR FEHDDDIKEE FFFSASLPTN TTSSELYEAV KNYIVNKCGL EFKFCVGVCS DGAASMTGKH SEVVTQIKEL APECKTTHCF IHRESLAMKK ISAELNSVLN DIVKIVNYIK SNSLNSRLFS LLCDNMEADH KQLLLHAEIR WLSRGKVLSR MFEIRNELLV FLQGKKPMWS QLFKDVNWTA RLAYLSDIFS IFNDLNASMQ GKNATYFSMA DKVEGQKQKL EAWKNRISTD CYDMFHNLTT IINEVGNDLD IAHLRKVISE HLTNLLECFE FYFPSKEDPR IGNLWIQNPF LSSKDNLNLT VTLQDKLLKL ATDEGLKISF ENTASLPSFW IKAKNDYPEL AEIALKLLLL FPSTYLCETG FSTLSVIKTK HRNSLNIHYP LRVALSSIQP RLDKLTSKKQ AHLSH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Grade
- Crystallography grade
-
-
- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
-
- Target
- SCAND3 (SCAN Domain Containing 3 (SCAND3))
- Alternative Name
- SCAND3 (SCAND3 Products)
- Synonyms
- Buster4 Protein, ZBED9 Protein, ZFP38-L Protein, ZNF305P2 Protein, ZNF452 Protein, dJ1186N24.3 Protein, SCAND3 Protein, zinc finger BED-type containing 9 Protein, SCAN domain containing 3 Protein, ZBED9 Protein, SCAND3 Protein
- Background
- SCAN domain-containing protein 3 (Transposon-derived Buster4 transposase-like protein) (Zinc finger BED domain-containing protein 9)
- Molecular Weight
- 151.7 kDa
- UniProt
- Q6R2W3
-
You are here:
