STAG3 Protein (AA 1-1225) (Strep Tag)
Crystallography grade
STAG3
Origin: Human
Host: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA
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- Target See all STAG3 products
- STAG3 (Stromal Antigen 3 (STAG3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1225
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Origin
- Human
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This STAG3 protein is labelled with Strep Tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- Sequence
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MSSPLQRAVG DTKRALSASS SSSASLPFDD RDSNHTSEGN GDSLLADEDT DFEDSLNRNV KKRAAKRPPK TTPVAKHPKK GSRVVHRHSR KQSEPPANDL FNAVKAAKSD MQSLVDEWLD SYKQDQDAGF LELVNFFIQS CGCKGIVTPE MFKKMSNSEI IQHLTEQFNE DSGDYPLIAP GPSWKKFQGS FCEFVRTLVC QCQYSLLYDG FPMDDLISLL TGLSDSQVRA FRHTSTLAAM KLMTSLVKVA LQLSVHQDNN QRQYEAERNK GPGQRAPERL ESLLEKRKEL QEHQEEIEGM MNALFRGVFV HRYRDVLPEI RAICIEEIGC WMQSYSTSFL TDSYLKYIGW TLHDKHREVR LKCVKALKGL YGNRDLTTRL ELFTSRFKDR MVSMVMDREY DVAVEAVRLL ILILKNMEGV LTDADCESVY PVVYASHRGL ASAAGEFLYW KLFYPECEIR MMGGREQRQS PGAQRTFFQL LLSFFVESEL HDHAAYLVDS LWDCAGARLK DWEGLTSLLL EKDQNLGDVQ ESTLIEILVS SARQASEGHP PVGRVTGRKG LTSKERKTQA DDRVKLTEHL IPLLPQLLAK FSADAEKVTP LLQLLSCFDL HIYCTGRLEK HLELFLQQLQ EVVVKHAEPA VLEAGAHALY LLCNPEFTFF SRADFARSQL VDLLTDRFQQ ELEELLQSSF LDEDEVYNLA ATLKRLSAFY NTHDLTRWEL YEPCCQLLQK AVDTGEVPHQ VILPALTLVY FSILWTLTHI SKSDASQKQL SSLRDRMVAF CELCQSCLSD VDTEIQEQAF VLLSDLLLIF SPQMIVGGRD FLRPLVFFPE ATLQSELASF LMDHVFIQPG DLGSGDSQED HLQIERLHQR RRLLAGFCKL LLYGVLEMDA ASDVFKHYNK FYNDYGDIIK ETLTRARQID RSHCSRILLL SLKQLYTELL QEHGPQGLNE LPAFIEMRDL ARRFALSFGP QQLQNRDLVV MLHKEGIQFS LSELPPAGSS NQPPNLAFLE LLSEFSPRLF HQDKQLLLSY LEKCLQHVSQ APGHPWGPVT TYCHSLSPVE NTAETSPQVL PSSKRRRVEG PAKPNREDVS SSQEESLQLN SIPPTPTLTS TAVKSRQPLW GLKEMEEEDG SELDFAQGQP VAGTERSRFL GPQYFQTPHN PSGPGLGNQL MRLSLMEEDE EEELEIQDES NEERQDTDMQ ASSYSSTSER GLDLLDSTEL DIEDF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- STAG3 (Stromal Antigen 3 (STAG3))
- Alternative Name
- STAG3 (STAG3 Products)
- Synonyms
- STAG3 Protein, SA-2 Protein, stromal antigen 3 Protein, STAG3 Protein, stag3 Protein, Stag3 Protein
- Background
- Cohesin subunit SA-3 (SCC3 homolog 3) (Stromal antigen 3) (Stromalin-3),FUNCTION: Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I. {ECO:0000269|PubMed:31682730}.
- Molecular Weight
- 139.0 kDa
- UniProt
- Q9UJ98
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