VPS8 Protein (AA 1-1428) (Strep Tag)

Catalog No. ABIN3096378

Product Details

Target: VPS8 (Vacuolar Protein Sorting 8 Homolog (VPS8))

Protein Type: Recombinant

Protein Characteristics: AA 1-1428

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This VPS8 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MENEPDHENV EQSLCAKTSE EELNKSFNLE ASLSKFSYID MDKELEFKND LIDDKEFDIP QVDTPPTLES ILNETDDEDE SFILEDPTLL NIDTIDSHSY DTSSVASSDS GDRTNLKRKK KLPDSFSLHG SVMRHSLLKG ISAQIVSAAD KVDAGLPTAI AVSSLIAVGT SHGLALIFGK DQNQALRLCL GSTSVGGQYG AISALSINND CSRLLCGFAK GQITMWDLAS GKLLRSITDA HPPGTAILHI KFTDDPTLAI CNDSGGSVFE LTFKRVMGVR TCESRCLFSG SKGEVCCIEP LHSKPELKDH PITQFSLLAM ASLTKILVIG LKPSLKVWMT FPYGRMDPSS VPLLAWHFVA VQNYVNPMLA FCRGDVVHFL LVKRDESGAI HVTKQKHLHL YYDLINFTWI NSRTVVLLDS VEKLHVIDRQ TQEELETVEI SEVQLVYNSS HFKSLATGGN VSQALALVGE KACYQSISSY GGQIFYLGTK SVYVMMLRSW RERVDHLLKQ DCLTEALALA WSFHEGKAKA VVGLSGDASK RKAIVADRMV EILFHYADRA LKKCPDQGKI QVMEQHFQDM VPVIVDYCLL LQRKDLLFSQ MYDKLSENSV AKGVFLECLE PYILSDKLVG ITPQVMKDLI VHFQDKKLME NVEALIVHMD ITSLDIQQVV LMCWENRLYD AMIYVYNRGM NEFISPMEKL FRVIAPPLNA GKTLTDEQVV MGNKLLVYIS CCLAGRAYPL GDIPEDLVPL VKNQVFEFLI RLHSAEASPE EEIYPYIRTL LHFDTREFLN VLALTFEDFK NDKQAVEYQQ RIVDILLKVM VENSDFTPSQ VGCLFTFLAR QLAKPDNTLF VNRTLFDQVL EFLCSPDDDS RHSERQQVLL ELLQAGGIVQ FEESRLIRMA EKAEFYQICE FMYEREHQYD KIIDCYLRDP LREEEVFNYI HNILSIPGHS AEEKQSVWQK AMDHIEELVS LKPCKAAELV ATHFSGHIET VIKKLQNQVL LFKFLRSLLD PREGIHVNQE LLQISPCITE QFIELLCQFN PTQVIETLQV LECYRLEETI QITQKYQLHE VTAYLLEKKG DIHGAFLIML ERLQSKLQEV THQGENTKED PSLKDVEDTM VETIALCQRN SHNLNQQQRE ALWFPLLEAM MAPQKLSSSA IPHLHSEALK SLTMQVLNSM AAFIALPSIL QRILQDPVYG KGKLGEIQGL ILGMLDTFNY EQTLLETTTS LLNQDLHWSL CNLRASVTRG LNPKQDYCSI CLQQYKRRQE MADEIIVFSC GHLYHSFCLQ NKECTVEFEG QTRWTCYKCS SSNKVGKLSE NSSEIKKGRI TPSQVKMSPS YHQSKGDPTA KKGTSEPVLD PQQIQAFDQL CRLYRGSSRL ALLTELSQNR SSESYRPFSG SQSAPAFNSI FQNENFQLQL IPPPVTED
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: VPS8 (Vacuolar Protein Sorting 8 Homolog (VPS8))

Alternative Name: VPS8 (VPS8 Products)

Synonyms: si:dkey-24b15.1 Protein, zgc:158695 Protein, KIAA0804 Protein, AI315068 Protein, AU040738 Protein, mKIAA0804 Protein, RGD1309443 Protein, VPS8, CORVET complex subunit Protein, vacuolar protein sorting 8 homolog (S. cerevisiae) Protein, VPS8 CORVET complex subunit Protein, vps8 Protein, VPS8 Protein, Vps8 Protein

Background: Vacuolar protein sorting-associated protein 8 homolog,FUNCTION: Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:25266290). Functions predominantly in APPL1-containing endosomes (PubMed:25266290). {ECO:0000269|PubMed:25266290, ECO:0000305|PubMed:25266290}.

Molecular Weight: 161.8 kDa

UniProt: Q8N3P4