ZNF326 Protein (AA 1-582) (Strep Tag)

Catalog No. ABIN3096487

Product Details

Target: ZNF326 (Zinc Finger Protein 326 (ZNF326))

Protein Type: Recombinant

Protein Characteristics: AA 1-582

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ZNF326 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MDFEDDYTHS ACRNTYQGFN GMDRDYGPGS YGGMDRDYGH GSYGGQRSMD SYLNQSYGMD NHSGGGGGSR FGPYESYDSR SSLGGRDLYR SGYGFNEPEQ SRFGGSYGGR FESSYRNSLD SFGGRNQGGS SWEAPYSRSK LRPGFMEDRG RENYSSYSSF SSPHMKPAPV GSRGRGTPAY PESTFGSRNY DAFGGPSTGR GRGRGHMGDF GSIHRPGIVV DYQNKSTNVT VAAARGIKRK MMQPFNKPSG TFIKKPKLAK PMEKISLSKS PTKTDPKNEE EEKRRIEARR EKQRRRREKN SEKYGDGYRM AFTCSFCKFR TFEEKDIELH LESSSHQETL DHIQKQTKFD KVVMEFLHEC MVNKFKKTSI RKQQTNNQTE VVKIIEKDVM EGVTVDDHMM KVETVHCSAC SVYIPALHSS VQQHLKSPDH IKGKQAYKEQ IKRESVLTAT SILNNPIVKA RYERFVKGEN PFEIQDHSQD QQIEGDEEDE EKIDEPIEEE EDEDEEEEAE EVGEVEEVEE VEEVREGGIE GEGNIQGVGE GGEVGVVGEV EGVGEVEEVE ELEEETAKEE PADFPVEQPE EN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ZNF326 (Zinc Finger Protein 326 (ZNF326))

Alternative Name: ZNF326 (ZNF326 Products)

Synonyms: ZNF326 Protein, zan75 Protein, zfp326 Protein, zird Protein, si:dkey-238n5.2 Protein, ZAN75 Protein, ZIRD Protein, Zfp326 Protein, dJ871E2.1 Protein, 5730470H14Rik Protein, Znf326 Protein, zinc finger protein 326 Protein, ZNF326 Protein, znf326 Protein, Zfp326 Protein

Background: DBIRD complex subunit ZNF326 (Zinc finger protein 326) (Zinc finger protein interacting with mRNPs and DBC1),FUNCTION: Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. May play a role in neuronal differentiation and is able to bind DNA and activate expression in vitro. {ECO:0000269|PubMed:22446626}.

Molecular Weight: 65.7 kDa

UniProt: Q5BKZ1