ZNF521 Protein (AA 1-1311) (Strep Tag)

Catalog No. ABIN3117396

Product Details

Target: ZNF521 (Zinc Finger Protein 521 (ZNF521))

Protein Type: Recombinant

Protein Characteristics: AA 1-1311

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ZNF521 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MSRRKQAKPR SLKDPNCKLE DKTEDGEALD CKKRPEDGEE LEDEAVHSCD SCLQVFESLS DITEHKINQC QLTDGVDVED DPTCSWPASS PSSKDQTSPS HGEGCDFGEE EGGPGLPYPC QFCDKSFSRL SYLKHHEQSH SDKLPFKCTY CSRLFKHKRS RDRHIKLHTG DKKYHCSECD AAFSRSDHLK IHLKTHTSNK PYKCAICRRG FLSSSSLHGH MQVHERNKDG SQSGSRMEDW KMKDTQKCSQ CEEGFDFPED LQKHIAECHP ECSPNEDRAA LQCVYCHELF VEETSLMNHM EQVHSGEKKN SCSICSESFH TVEELYSHMD SHQQPESCNH SNSPSLVTVG YTSVSSTTPD SNLSVDSSTM VEAAPPIPKS RGRKRAAQQT PDMTGPSSKQ AKVTYSCIYC NKQLFSSLAV LQIHLKTMHL DKPEQAHICQ YCLEVLPSLY NLNEHLKQVH EAQDPGLIVS AMPAIVYQCN FCSEVVNDLN TLQEHIRCSH GFANPAAKDS NAFFCPHCYM GFLTDSSLEE HIRQVHCDLS GSRFGSPVLG TPKEPVVEVY SCSYCTNSPI FNSVLKLNKH IKENHKNIPL ALNYIHNGKK SRALSPLSPV AIEQTSLKMM QAVGGAPARP TGEYICNQCG AKYTSLDSFQ THLKTHLDTV LPKLTCPQCN KEFPNQESLL KHVTIHFMIT STYYICESCD KQFTSVDDLQ KHLLDMHTFV FFRCTLCQEV FDSKVSIQLH LAVKHSNEKK VYRCTSCNWD FRNETDLQLH VKHNHLENQG KVHKCIFCGE SFGTEVELQC HITTHSKKYN CKFCSKAFHA IILLEKHLRE KHCVFETKTP NCGTNGASEQ VQKEEVELQT LLTNSQESHN SHDGSEEDVD TSEPMYGCDI CGAAYTMETL LQNHQLRDHN IRPGESAIVK KKAELIKGNY KCNVCSRTFF SENGLREHMQ THLGPVKHYM CPICGERFPS LLTLTEHKVT HSKSLDTGNC RICKMPLQSE EEFLEHCQMH PDLRNSLTGF RCVVCMQTVT STLELKIHGT FHMQKTGNGS AVQTTGRGQH VQKLYKCASC LKEFRSKQDL VKLDINGLPY GLCAGCVNLS KSASPGINVP PGTNRPGLGQ NENLSAIEGK GKVGGLKTRC SSCNVKFESE SELQNHIQTI HRELVPDSNS TQLKTPQVSP MPRISPSQSD EKKTYQCIKC QMVFYNEWDI QVHVANHMID EGLNHECKLC SQTFDSPAKL QCHLIEHSFE GMGGTFKCPV CFTVFVQANK LQQHIFSAHG QEDKIYDCTQ CPQKFFFQTE LQNHTMTQHS S
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ZNF521 (Zinc Finger Protein 521 (ZNF521))

Alternative Name: ZNF521 (ZNF521 Products)

Synonyms: Znf521 Protein, EHZF Protein, Evi3 Protein, B930086A16Rik Protein, zinc finger protein 521 Protein, zinc finger protein 521 S homeolog Protein, Zfp521 Protein, ZNF521 Protein, znf521.S Protein

Background: Zinc finger protein 521 (Early hematopoietic zinc finger protein) (LYST-interacting protein 3),FUNCTION: Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage, this interaction preventing EBF1 to bind DNA and activate target genes. {ECO:0000269|PubMed:14630787}.

Molecular Weight: 147.9 kDa

UniProt: Q96K83