Aquaporin 4 Protein (AQP4) (AA 1-323) (rho-1D4 tag,His tag)

Catalog No. ABIN3089096

Product Details

Target: Aquaporin 4 (AQP4)

Protein Type: Recombinant

Protein Characteristics: AA 1-323

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This Aquaporin 4 protein is labelled with rho-1D4 tag,His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: MTETSQVAPA GSSGHHHHHH SDRPTARRWG KCGPLCTREN IMVAFKGVWT QAFWKAVTAE FLAMLIFVLL SLGSTINWGG TEKPLPVDMV LISLCFGLSI ATMVQCFGHI SGGHINPAVT VAMVCTRKIS IAKSVFYIAA QCLGAIIGAG ILYLVTPPSV VGGLGVTMVH GNLTAGHGLL VELIITFQLV FTIFASCDSK RTDVTGSIAL AIGFSVAIGH LFAINYTGAS MNPARSFGPA VIMGNWENHW IYWVGPIIGA VLAGGLYEYV FCPDVEFKRR FKEAFSKAAQ QTKGSYMEVE DNRSQVETDD LILKPGVVHV IDVDRGEEKK GKDQSGEVLS SV
Sequence including N-terminal Rho1D4 tag and N-terminal His-tag.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human AQP4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. The protein is purified from the cleared cell lysate using His-tag capture materials.
2. Eluate fractions are analyzed by SDS-PAGE.
3. Protein containing fractions are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In solution (20 mM Hepes, pH 8.0, 150 mM NaCl, 0,8 % Octylglucopyranoside)

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Aquaporin 4 (AQP4)

Alternative Name: AQP4 (AQP4 Products)

Synonyms: zgc:100922 Protein, si:ch211-192k9.1 Protein, AQP4 Protein, LOC100232616 Protein, HMIWC2 Protein, MIWC Protein, WCH4 Protein, AQP-4 Protein, AQP4.M23 Protein, MIWC2 Protein, Miwc Protein, aquaporin-4 Protein, aquaporin 4 Protein, aquaporin 4 L homeolog Protein, aqp4 Protein, AQP4 Protein, aqp4.L Protein, Aqp4 Protein

Background: Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

Molecular Weight: 36.8 kDa Including tag.

UniProt: P55087

Pathways: Sensory Perception of Sound