ARPC1B Protein (AA 2-372) (His tag)
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- Target See all ARPC1B Proteins
- ARPC1B (Actin Related Protein 2/3 Complex, Subunit 1B, 41kDa (ARPC1B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-372
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This ARPC1B protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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AYHSFLVEPI SCHAWNKDRT QIAICPNNHE VHIYEKSGAK WNKVHELKEH NGQVTGIDWA PESNRIVTCG TDRNAYVWTL KGRTWKPTLV ILRINRAARC VRWAPNENKF AVGSGSRVIS ICYFEQENDW WVCKHIKKPI RSTVLSLDWH PNNVLLAAGS CDFKCRIFSA YIKEVEERPA PTPWGSKMPF GELMFESSSS CGWVHGVCFS ASGSRVAWVS HDSTVCLVDA DKKMAVATLA SETLPLLAVT FITENSLVAA GHDCFPVLFT YDNAAVTLSF GGRLDVPKQS SQRGMTARER FQNLDKKASS EGGAATGAGL DSLHKNSVSQ ISVLSGGKAK CSQFCTTGMD GGMSIWDVKS LESALKDLKI K
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Arpc1b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product ARPC1B Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ARPC1B (Actin Related Protein 2/3 Complex, Subunit 1B, 41kDa (ARPC1B))
- Alternative Name
- Arpc1b (ARPC1B Products)
- Synonyms
- ARC41 Protein, p40-ARC Protein, p41-ARC Protein, cb871 Protein, arpc1a Protein, fj15h02 Protein, zgc:64056 Protein, wu:fj08e12 Protein, wu:fj15h02 Protein, 41kDa Protein, AA408064 Protein, AA408534 Protein, AA571392 Protein, AF007010 Protein, AW208418 Protein, L72 Protein, SOP2Hs Protein, ACTIN-RELATED PROTEIN C1 Protein, ARPC1 Protein, F16D14.14 Protein, F16D14_14 Protein, actin-related protein C1B Protein, arc41 Protein, p40-arc Protein, p41-arc Protein, actin related protein 2/3 complex subunit 1B Protein, actin related protein 2/3 complex, subunit 1B Protein, ARP2/3 complex 41 kDa subunit Protein, actin-related protein C1B Protein, actin-related protein 2/3 complex subunit 1B Protein, actin related protein 2/3 complex subunit 1B S homeolog Protein, ARPC1B Protein, Arpc1b Protein, EHI_094120 Protein, arpc1b Protein, LOC100410640 Protein, arpc1b.S Protein
- Background
- Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. {ECO:0000250}.
- Molecular Weight
- 41.9 kDa Including tag.
- UniProt
- Q9WV32
- Pathways
- RTK Signaling, Regulation of Actin Filament Polymerization
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