IQUB Protein (AA 1-788) (Strep Tag)

Catalog No. ABIN3125893

Product Details

Target: IQUB (IQ Motif and Ubiquitin Domain Containing (IQUB))

Protein Type: Recombinant

Protein Characteristics: AA 1-788

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This IQUB protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSDPEEERVA DSTAHYEEAG KVISIPRPSD EAEGSDVMPE QDDEVQELTT ESEENVERHS EFSLSTPKSD DSKPREEVTS LGSASGSQDQ DYRLLDYQKG EDDELLFLHK IKAIKETLQT SVRESIATVK IVLIPAGQEI IMPFRVDAPF RFLKEHFAHL LHIPHYVLQI THEGIIVGNN ESLIQYGIKP QEIVQVEVFS TLPDQYPVRR IEGLSEGSQI ITVTIQTSID RYEEVAVEII KSDFHKPFLG GFRHKITGLE YHNAGTQTVP RKIPEKDNLF CRDTQTVFQK KKLQQTTNTT STQMTKIGVY VSNMTDKLLK PGNYFSAAEY HARRLHAVIV IQTSYRRWHA KRYVESLRKQ KKLRLEWEEE QELLKIQEKE EWIRMDYYRR HNPKTTEDFE LLYNALELWR QEEVEQICHY SSEAERKAAL CELLEKETQM IASIGRHRSA ARMERQDAAI QAFLDKCSAP NVWRRGDGKT IEMDTQFTIR ARELQSIYKC ILLKDLSQDE RLDILLTLKH TVKEHECKLT QEILELIDRE VDLMMRGVKP HNLEGLRKRI TTLFIHYIKT PLFNPEVAKY LKVPQDPLKF YDTIYFCHSC QNYLPSVEFS VSPTSHRVYR CRHCINLENE TQRRESFLKY KCLLQRLYYS EADYGDNSQI AFLMQLQDIK YLTENIWASQ SALSAWDDLN DLVMVRWDKH VEWSPWNCIL LTKDESTAHL RLPSIEKGYG HHFVHKIKHK HILAKNYFSQ IPTLASLILN DDEVEDIRSK HSTKSPPKII ITRRIQPH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: IQUB (IQ Motif and Ubiquitin Domain Containing (IQUB))

Alternative Name: Iqub (IQUB Products)

Synonyms: 4932408B21Rik Protein, Trs4 Protein, RGD1562359 Protein, IQ motif and ubiquitin domain containing Protein, IQUB Protein, Iqub Protein

Background: IQ motif and ubiquitin-like domain-containing protein,FUNCTION: Anchors the radial spoke 1 (RS1) complex to the A microtubule of outer doublet microtubules in axonemes (PubMed:36417862, PubMed:36355624). The triple radial spokes (RS1, RS2 and RS3) are required to modulate beating of the sperm flagellum (PubMed:36417862, PubMed:36355624). May play a role in inhibiting signaling via MAPK1/ERK2 and MAPK3/ERK1 (PubMed:36355624). Additionally, may play a role in the functioning of cilia (PubMed:21289087). Not required for the functioning of tracheal or ependymal cilia (PubMed:36417862). {ECO:0000269|PubMed:21289087, ECO:0000269|PubMed:36355624, ECO:0000269|PubMed:36417862}.

Molecular Weight: 92.1 kDa

UniProt: Q8CDK3