DENND2D Protein (AA 1-469) (Strep Tag)
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- Target See all DENND2D products
- DENND2D (DENN/MADD Domain Containing 2D (DENND2D))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-469
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This DENND2D protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MEGQGVGRTL RLLRNRLPRL RAGQSQNNPG EAVTEPERIQ EHSPSSFAGG QHFFEYLLVV SLKKKRLGDD YEPTITYQFP KRENLLRGQQ EEEDRLLSAI PLFCFPDGNE WAPLTEYPRE TFSFVLTNVD GSRKIGYCRR LLPAGPGPQL PKVYCIISCI GCFGLFSKIL DEVEKRHQIS MAVIYPFMQG LREAAFPAPG KTVTLKSFIP DSGTEFISLT RPLDSHLEHV DFSVLLHCLH LEQIIQIFAS AVLERKIIFL AEGLSTLSQC IHAAAALLYP FSWAHTYIPV VPESLLATVC CPTPFMVGVQ MRFLQEVMDS PMEEVLLVNL CEGTFLLSVG DEKDILPPKL QDDILDSLGQ GINELKTSEQ INEHVSGPFV QFFVKTVGHY ASYIKREASG QGHFQERSFC KAVTSKTKRR FVKKFVKTQL FSLFIQEAEK SRNPPAGYFQ KKILEYEEQK KQKKSRERL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- DENND2D (DENN/MADD Domain Containing 2D (DENND2D))
- Alternative Name
- Dennd2d (DENND2D Products)
- Synonyms
- 2010308M01Rik Protein, AI451421 Protein, RGD1305120 Protein, MGC84588 Protein, dennd2d Protein, zgc:103487 Protein, zgc:153628 Protein, DENN/MADD domain containing 2D Protein, DENN domain containing 2D Protein, DENN/MADD domain containing 2D S homeolog Protein, DENN/MADD domain containing 2Db Protein, DENN/MADD domain containing 2Da Protein, Dennd2d Protein, DENND2D Protein, dennd2d.S Protein, dennd2db Protein, dennd2da Protein, dennd2d Protein
- Background
- DENN domain-containing protein 2D,FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (By similarity). {ECO:0000250}.
- Molecular Weight
- 53.2 kDa
- UniProt
- Q91VV4
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