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Ribophorin II Protein (RPN2) (AA 23-540) (His tag)

Crystallography grade RPN2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Catalog No. ABIN3129555
  • Target See all Ribophorin II (RPN2) Proteins
    Ribophorin II (RPN2)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 23-540
    Origin
    • 5
    • 1
    Mouse
    Source
    • 2
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This Ribophorin II protein is labelled with His tag.
    Application
    Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
    Sequence
    LTPTHYLTKQ DVERLKASLD RPFTDLESAF YSIVGLSSLG VQVPDVKKAC TFIKSNLDPS NVDSLFYAAQ SSQVLSGCEI SVSNETKELL LAAVSEDSPI AQIYHAVAAL SGFGLPLASN EALGALTARL GKEETVLATV QALQTASHLS QQADLRNIVE EIEDLVARLD ELGGVYLQFE EGLELTALFV AATYKLMDHV GTEPSMKEDQ VIQLMNTIFS KKNFESLSEA FSVASAAAAL SQNRYHVPVV VVPEGSTSDT QEQAILRLQV SNVLSQPLAQ AAVKLEHAKS AATRATVLQK TPFSLVGNVF ELNFKNVKLS SGYYDFSVRV EGDSRYIANT VELRVKISTE VGITNVDLST VDKDQSIAPK TTRVTYPAKA KGTFIADSHQ NFALFFQLVD VNTGAELTPH QTFVRLHNQK TGQEVVFVAE PDNKNVYKFE LDTSERKIEF DSASGTYTLY LIIGDATLKN PILWNVADVV IKFPEEEAPS TVLSQSLFTP KQEIQHLFRE PEKRPPTV
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Rpn2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product RPN2 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Ribophorin II (RPN2)
    Alternative Name
    Rpn2 (RPN2 Products)
    Synonyms
    RIBIIR Protein, RPN-II Protein, RPNII Protein, SWP1 Protein, RPN2 Protein, 1300012C06Rik Protein, AV261018 Protein, Rpn-2 Protein, fb95d11 Protein, fj62b10 Protein, wu:fb61f08 Protein, wu:fb74e07 Protein, wu:fb95d11 Protein, wu:fj41f11 Protein, wu:fj62b10 Protein, rpn2 Protein, ribophorin II Protein, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 Protein, ribophorin II S homeolog Protein, RPN2 Protein, Rpn2 Protein, rpn2 Protein, LOC5579328 Protein, Bm1_30625 Protein, rpn2.S Protein
    Background
    Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. {ECO:0000250}.
    Molecular Weight
    57.8 kDa Including tag.
    UniProt
    Q9DBG6
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