Gm1564 (AA 1-965) protein (Strep Tag)
Crystallography grade
Origin: Mouse
Host: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB
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- Target
- Gm1564
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-965
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- Strep Tag
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MEVSGGDTCR PRHPQGLREG PEPKVAAAAA AFRGSANRCW NLSVDTSNRL SDVFNSMMLT GSAPFYDCYK SQNEDNVDLR QTCTPLSSST EYASSIDSSL FYAPWSTYGD DIKQPPSSQI SVKNRIQTER NDYGSETDLY GLVSNILEEQ DKSQPYFAEG TCSSNLKSVW PMNTSRFVDH HDLLTEPKRP VDTSISQQAF YSGESVSAVE KQYLHNSSLT PQQKIDELYH GYTGLDLEEQ WLYLSRSDHS NCYNSQANDT VKATFQEYPF VKNCFTPQTG LSDIMKESGI DTYAYGREKI CTKGLETPLQ HKRAEIFLSQ FNRYNENADY CRYPEYAHPN KAKLNKCSNF SVQDGKKLAN GTPETPTVEA DAYTKLFQVK PANQKKMEET IPDQQNFAFP KTTPHLTEKQ FAKEAAFTAD FGLKSEYGLK PHTACPTNND FANVSEKQQF AKPDPLNSEY FKSVNLFSNS ATSSGGISLN RPTWMNVQTK NNLPIPYRNQ GNLMKLNSHL SAASKGSNHS SDFPQLSSTN LTSNSNLFQK YCQENPSAFS SFDFSYNGAE RIQSVNHMEG LTKTGEDNLF ESVTEKKIKQ PNGFCDSYSA SQYGIIENVN KHNFQAKPQS GHYDPEDIPK HFDGLPQNTY QDLLESQGHF NSHRQGSGDN NINSRVNRTQ ASCFSNNYMM GDLRHNQGFQ QLGSNGFPLR STHPFGHSVV PLLDSYDLFS YDDLSHLYPY FNDMMYGDNS FSGFVPTFGF QRPIKTRSGP ASELHIRLEE CYEQWRALEK ERKKTELALA KNYPGKKVSS TNNTPIPRLT SNPSRVDRLI VDELREQARV VTLLGKMERL RSSPLHANIS TALDRHLESI HIVQSRRKDE IVNASNRQRQ GVPRCQDDRD VFALATAIKE MCVATRKART TLWCALQMTL PKTASTAGQA DMEKAFQDLV NCEEKVHESI NSSNPMNQRG ETSKH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Gm1564
- Alternative Name
- Meioc
- Synonyms
- Gm663 Protein, meiosis specific with coiled-coil domain Protein, Meioc Protein
- Background
- Meiosis-specific coiled-coil domain-containing protein MEIOC (Meiosis-specific with coiled-coil domain protein),FUNCTION: Is required for meiosis completion in both male and female germ cells. Confers stability to numerous meiotic mRNAs in gonads allowing proper initiation and progression into meiosis prophase I. The function may involve YTHDC2 and is independent of induction by retinoic acid (RA). Maintains an extended meiotic prophase I by properly promoting the transition from a mitotic to a meiotic cell cycle program by binding transcripts through its interaction with YTHDC2 that regulate the mitotic cell cycle (PubMed:28380054). {ECO:0000269|PubMed:26742488, ECO:0000269|PubMed:28380054}.
- Molecular Weight
- 108.8 kDa
- UniProt
- A2AG06
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