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G Protein-Coupled Receptor 116 Protein (GPR116) (AA 22-1019) (His tag)

Crystallography grade GPR116 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3131217
$24,004.89
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
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  • Target See all G Protein-Coupled Receptor 116 (GPR116) Proteins
    G Protein-Coupled Receptor 116 (GPR116)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 22-1019
    Origin
    • 3
    • 1
    Mouse
    Source
    • 3
    • 1
    Insect Cells
    Purification tag / Conjugate
    This G Protein-Coupled Receptor 116 protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    ALSTPTEPIV QPSILQEHEL AGEELLRPKR AAAAGDRVAE EYMVDIEISF ENVSFLESIR AHLNNLSFPI RGTEADILNI AMTTVCTPAG NDLLCFCEKG YQWSEERCLH SLTCQDYDSA LPGGYCSCLK GLPPQGPFCQ LPEAFITLKL KVRLNIGFQE DLKNTSSALY RSYKTDLERA FRAGYRTLPG FRSVTVTQFT KGSVVVNYVV RVTSAPLPGS IHKANEQVIQ NLNHTYKMDY NSFQGTPSNE TKFTVIPEFI FEGDNVTLEC ETEFVTSNTS WYYGEKRSDI QNSDKYSIHT TVINNISLIT RLTIYNFTQH DAGMYGCNVT LDIFEYGTVR KLDVTPIRIL AKEERKVVCD NHPISLNCCS ENIANWSSIE WKQEGKISIL GNPESDLESS CSTYTLKADG TQCPSGSSGT TVIYTCEFVS AYGARGSKNI AVTFTSVANL TITRDPISVS EGQSFSITCL SDVSSFDEVY WNTSAGIKIH PRFYTMRRYQ DGAESVLMVK TSTREWNGTY HCIFRYKNSY SIATKDVTVH PLPLVSDIMM DPLEASGLCT SSHQFKCCVE EDAGEEYAVT FHVDSSSFPA EREVIGKQAC YTYSLPANLP RCPKDIAVFC HFTNAANSSV RSPSMKLKLI PRENVTCQDP IIGIGDPGKV IQKLCQFSGV YGSPGQAIGG TVTYKCVGTQ WKEESRACIS APINGLLQVA KALIKSPTQD QKLPTYLRDL SVSAGKEEQD IRSSPGSLGA IISILDLLST VPTQVNSEMM RDILATINVI LDKSALNSWE KLLQQQSNQS SQFLHSVERF SQALQLGDST PPFLAHPNVQ MKSMVIKRGH PQIYQQQFIF KDSDLWGDVA IDECQLGNLQ PDSSIVTVAF PTLKAILAQD VQRKTSSNSL VMTTTVSHNI VKPFRISMTF KNNHRSGGKP QCVFWNFSLA NNTGGWDSSG CSVEDDGRDN RDRVFCKCNH LTSFSILMSP DSPDPGSLLK ILLDIISY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Adgrf5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    G Protein-Coupled Receptor 116 (GPR116)
    Alternative Name
    Adgrf5 (GPR116 Products)
    Synonyms
    GPR116 Protein, KPG_001 Protein, 8430401C09Rik Protein, 9330185D23 Protein, AI528491 Protein, mKIAA0758 Protein, Gprhep Protein, Ig-Hepta Protein, G protein-coupled receptor 116 Protein, adhesion G protein-coupled receptor F5 Protein, adhesion G protein-coupled receptor F1 Protein, GPR116 Protein, ADGRF5 Protein, adgrf1 Protein, Adgrf5 Protein
    Background
    Receptor that plays a critical role in lung surfactant homeostasis (PubMed:23590306, PubMed:23922714, PubMed:23684610). May play a role in controlling adipocyte function (PubMed:22971422). {ECO:0000269|PubMed:22971422, ECO:0000269|PubMed:23590306, ECO:0000269|PubMed:23684610, ECO:0000269|PubMed:23922714}.
    Molecular Weight
    111.6 kDa Including tag.
    UniProt
    G5E8Q8
    Pathways
    Carbohydrate Homeostasis
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