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HDAC1 Protein (AA 1-482) (Strep Tag)

HDAC1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). WB, SDS, ELISA
Catalog No. ABIN3131387
  • Target See all HDAC1 Proteins
    HDAC1 (Histone Deacetylase 1 (HDAC1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-482
    Origin
    • 11
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 10
    • 6
    • 2
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This HDAC1 protein is labelled with Strep Tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA
    Sequence
    MAQTQGTKRK VCYYYDGDVG NYYYGQGHPM KPHRIRMTHN LLLNYGLYRK MEIYRPHKAN AEEMTKYHSD DYIKFLRSIR PDNMSEYSKQ MQRFNVGEDC PVFDGLFEFC QLSTGGSVAS AVKLNKQQTD IAVNWAGGLH HAKKSEASGF CYVNDIVLAI LELLKYHQRV LYIDIDIHHG DGVEEAFYTT DRVMTVSFHK YGEYFPGTGD LRDIGAGKGK YYAVNYPLRD GIDDESYEAI FKPVMSKVME MFQPSAVVLQ CGSDSLSGDR LGCFNLTIKG HAKCVEFVKS FNLPMLMLGG GGYTIRNVAR CWTYETAVAL DTEIPNELPY NDYFEYFGPD FKLHISPSNM TNQNTNEYLE KIKQRLFENL RMLPHAPGVQ MQAIPEDAIP EESGDEDEED PDKRISICSS DKRIACEEEF SDSDEEGEGG RKNSSNFKKA KRVKTEDEKE KDPEEKKEVT EEEKTKEEKP EAKGVKEEVK LA
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Purity
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
    Top Product
    Discover our top product HDAC1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    HDAC1 (Histone Deacetylase 1 (HDAC1))
    Alternative Name
    Hdac1 (HDAC1 Products)
    Synonyms
    GON-10 Protein, HD1 Protein, RPD3 Protein, RPD3L1 Protein, CG7471 Protein, DHDAC1 Protein, DRpd3 Protein, DmHDAC1 Protein, Dmel\\CG7471 Protein, E(var)3-64BC Protein, HDAC Protein, HDAC-1 Protein, HDAC1 Protein, Hdac1 Protein, Rpd3/HDAC Protein, Su(var)3-26 Protein, Su(var)326 Protein, Su(var)328 Protein, dHDAC-1 Protein, dHDAC1 Protein, dRPD3 Protein, dRpd3 Protein, dmHDA401 Protein, drpd3 Protein, hdac1 Protein, l(3)04556 Protein, l(3)64Cc Protein, rpd3 Protein, rpd[3] Protein, gon-10 Protein, hdac1b Protein, rpd3l1 Protein, Rpd3 Protein, GB14706 Protein, hdac1-b Protein, chunp6919 Protein, hdac-1 Protein, mp:zf637-2-001987 Protein, wu:fb19h11 Protein, wu:fi06f03 Protein, zgc:101582 Protein, zgc:65818 Protein, Hdac1-ps Protein, MommeD5 Protein, ARABIDOPSIS HISTONE DEACETYLASE 1 Protein, ARABIDOPSIS HISTONE DEACETYLASE 19 Protein, ATHD1 Protein, ATHDA19 Protein, ATRPD3A Protein, F20D10.250 Protein, F20D10_250 Protein, HDA1 Protein, HDA19 Protein, HISTONE DEACETYLASE Protein, HISTONE DEACETYLASE 19 Protein, HISTONE DEACETYLASE19 Protein, RPD3A Protein, histone deacetylase 1 Protein, HDM Protein, ab21 Protein, hdac1a Protein, histone deacetylase 1 Protein, Histone deacetylase 1 Protein, histone deacetylase Protein, histone deacetylase 1 S homeolog Protein, histone deacetylase Rpd3 Protein, histone deacetylase 1 L homeolog Protein, HDAC1 Protein, hdac1.S Protein, LOC411503 Protein, hdac1 Protein, Hdac1 Protein, HD1 Protein, hda-1 Protein, hdac1.L Protein, LOC748850 Protein
    Background
    Histone deacetylase 1 (HD1) (EC 3.5.1.98) (Protein deacetylase HDAC1) (EC 3.5.1.-) (Protein decrotonylase HDAC1) (EC 3.5.1.-),FUNCTION: Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10615135, PubMed:15542849, PubMed:21960634, PubMed:30279482). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10615135, PubMed:15542849, PubMed:21960634). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10615135, PubMed:21960634). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (By similarity). As part of the SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription (By similarity). Also functions as a deacetylase for non-histone targets, such as NR1D2, RELA, SP1, SP3, STAT3 and TSHZ3 (By similarity). Deacetylates SP proteins, SP1 and SP3, and regulates their function (By similarity). Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons (By similarity). Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation (By similarity). Deacetylates TSHZ3 and regulates its transcriptional repressor activity (By similarity). Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B (By similarity). Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity (By similarity). Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (PubMed:17707228). Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer (PubMed:15226430, PubMed:24736997). Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation (PubMed:15226430). In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones (PubMed:30279482). {ECO:0000250|UniProtKB:Q13547, ECO:0000269|PubMed:10615135, ECO:0000269|PubMed:15226430, ECO:0000269|PubMed:15542849, ECO:0000269|PubMed:17707228, ECO:0000269|PubMed:21960634, ECO:0000269|PubMed:24736997, ECO:0000269|PubMed:30279482}.
    Molecular Weight
    55.1 kDa
    UniProt
    O09106
    Pathways
    Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis
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