SH3PXD2A Protein (AA 1-1124) (Strep Tag)

Catalog No. ABIN3132302

Product Details

Target: SH3PXD2A (SH3 and PX Domains 2A (SH3PXD2A))

Protein Type: Recombinant

Protein Characteristics: AA 1-1124

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SH3PXD2A protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MLAYCVQDAT VVDVEKRRSP SKHYVYIINV TWSDSTSQTI YRRYSKFFDL QMQLLDKFPI EGGQKDPKQR IIPFLPGKIL FRRSHIRDVA VKRLKPIDEY CRALVRLPPH ISQCDEVFRF FEARPEDVNP PKEDYGSSKR KSVWLSSWAE SPKKDVTGAD TNAEPMILEQ YVVVSNYKKQ ENSELSLQAG EVVDVIEKNE SGWWFVSTSE EQGWVPATYL EAQNGTRDDS DINTSKTGEV SKRRKAHLRR LDRRWTLGGM VNRQHSREEK YVTVQPYTSQ SKDEIGFEKG VTVEVIRKNL EGWWYIRYLG KEGWAPASYL KKAKDDLPTR KKNLAGPVEI IGNIMEISNL LNKKASGDKE APAEGEGSEA PITKKEISLP ILCNASNGSA LAIPERTTSK LAQGSPAVAR IAPQRAQISS PNLRTRPPPR RESSLGFQLP KPPEPPSVEV EYYTIAEFQS CISDGISFRG GQKAEVIDKN SGGWWYVQIG EKEGWAPASY IDKRKKPNLS RRTSTLTRPK VPPPAPPSKP KEAEENPVGA CESQGSPLKV KYEEPEYDVP AFGFDSEPEM NEEPSGDRGS GDKHPAQPRR ISPASSLQRA HFKVGESSED VALEEETIYE NEGFRPYTED TLSARGSSGD SDSPGSSSLS LAVKNSPKSD SPKSSSLLKL KAEKNAQAEL GKNQSNISFS SSVTISTTCS SSSSSSSLSK NNGDLKPRSA SDAGIRDTPK VGTKKDPDVK AGLASCARAK PSVRPKPVLN RAESQSQEKM DISSLRRQLR PTGQLRGGLK GSRSEDSELP PQMASEGSRR GSADIIPLTA TTPPCVPKKE WEGQGATYVT CSAYQKVQDS EISFPEGAEV HVLEKAESGW WYVRFGELEG WAPSHYLVAE ENQQPDTASK EGDTGKSSQN EGKSDSLEKI EKRVQALNTV NQSKRATPPI PSKPPGGFGK TSGTVAVKMR NGVRQVAVRP QSVFVSPPPK DNNLSCALRR NESLTATDSL RGVRRNSSFS TARSAAAEAK GRLAERAASQ GSESPLLPTQ RKGIPVSPVR PKPIEKSQFI HNNLKDVYIS IADYEGDEET AGFQEGVSME VLEKNPNGWW YCQILDEVKP FKGWVPSNYL EKKN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SH3PXD2A (SH3 and PX Domains 2A (SH3PXD2A))

Alternative Name: Sh3pxd2a (SH3PXD2A Products)

Synonyms: FISH Protein, SH3MD1 Protein, TKS5 Protein, 2310014D11Rik Protein, AA589508 Protein, AI256723 Protein, AI413738 Protein, C230050L11 Protein, EG329070 Protein, Fish Protein, Gm5098 Protein, Sh3md1 Protein, TSK5 Protein, Tks5 Protein, sh3pxd2a Protein, si:dkey-82d4.2 Protein, SH3 and PX domains 2A Protein, SH3 and PX domains 2Aa Protein, SH3PXD2A Protein, Sh3pxd2a Protein, sh3pxd2aa Protein

Background: SH3 and PX domain-containing protein 2A (Five SH3 domain-containing protein) (SH3 multiple domains protein 1) (Tyrosine kinase substrate with five SH3 domains),FUNCTION: Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide (By similarity). {ECO:0000250, ECO:0000269|PubMed:18417249, ECO:0000269|PubMed:19755709}.

Molecular Weight: 124.2 kDa

UniProt: O89032