GNA15 Protein (AA 1-374) (Strep Tag)
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- Target See all GNA15 products
- GNA15 (Guanine Nucleotide Binding Protein (G Protein), alpha 15 (Gq Class) (GNA15))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-374
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Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This GNA15 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MARSLTWGCC PWCLTEEEKT AARIDQEINR ILLEQKKQER EELKLLLLGP GESGKSTFIK QMRIIHGVGY SEEDRRAFRL LIYQNIFVSM QAMIDAMDRL QIPFSRPDSK QHASLVMTQD PYKVSTFEKP YAVAMQYLWR DAGIRACYER RREFHLLDSA VYYLSHLERI SEDSYIPTAQ DVLRSRMPTT GINEYCFSVK KTKLRIVDVG GQRSERRKWI HCFENVIALI YLASLSEYDQ CLEENDQENR MEESLALFST ILELPWFKST SVILFLNKTD ILEDKIHTSH LATYFPSFQG PRRDAEAAKS FILDMYARVY ASCAEPQDGG RKGSRARRFF AHFTCATDTQ SVRSVFKDVR DSVLARYLDE INLL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
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Guanine Nucleotide Binding Protein (G Protein), alpha 15 (Gq Class) (GNA15) (AA 1-374) protein (GST tag)
GNA15 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Guanine Nucleotide Binding Protein (G Protein), alpha 15 (Gq Class) (GNA15) protein (His tag)GNA15 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- GNA15 (Guanine Nucleotide Binding Protein (G Protein), alpha 15 (Gq Class) (GNA15))
- Alternative Name
- Gna15 (GNA15 Products)
- Synonyms
- GNA16 Protein, G[a]15 Protein, Galpha15 Protein, G protein subunit alpha 15 Protein, guanine nucleotide binding protein (G protein), alpha 15 (Gq class) Protein, guanine nucleotide binding protein, alpha 15 Protein, GNA15 Protein, Gna15 Protein
- Background
- Guanine nucleotide-binding protein subunit alpha-15 (G alpha-15) (G-protein subunit alpha-15),FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
- Molecular Weight
- 43.5 kDa
- UniProt
- P30678
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