PTPRE Protein (AA 69-699) (His tag)
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- Target See all PTPRE Proteins
- PTPRE (Protein tyrosine Phosphatase, Receptor Type, E (PTPRE))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 69-699
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This PTPRE protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequence
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RFRKQRKAVV SSNDKKMPNG ILEEQEQQRV MLLSRSPSGP KKFFPIPVEH LEEEIRVRSA DDCKRFREEF NSLPSGHIQG TFELANKEEN REKNRYPNIL PNDHCRVILS QVDGIPCSDY INASYIDGYK EKNKFIAAQG PKQETVNDFW RMVWEQRSAT IVMLTNLKER KEEKCYQYWP DQGCWTYGNI RVCVEDCVVL VDYTIRKFCI HPQLPDSCKA PRLVSQLHFT SWPDFGVPFT PIGMLKFLKK VKTLNPSHAG PIVVHCSAGV GRTGTFIVID AMMDMIHSEQ KVDVFEFVSR IRNQRPQMVQ TDVQYTFIYQ ALLEYYLYGD TELDVSSLER HLQTLHSTAT HFDKIGLEEE FRKLTNVRIM KENMRTGNLP ANMKKARVIQ IIPYDFNRVI LSMKRGQEFT DYINASFIDG YRQKDYFMAT QGPLAHTVED FWRMVWEWKS HTIVMLTEVQ EREQDKCYQY WPTEGSVTHG DITIEIKSDT LSEAISVRDF LVTFKQPLAR QEEQVRMVRQ FHFHGWPEVG IPAEGKGMID LIAAVQKQQQ QTGNHPITVH CSAGAGRTGT FIALSNILER VKAEGLLDVF QAVKSLRLQR PHMVQTLEQY EFCYKVVQDF IDIFSDYANF K
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Ptpre Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product PTPRE Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- PTPRE (Protein tyrosine Phosphatase, Receptor Type, E (PTPRE))
- Alternative Name
- Ptpre (PTPRE Products)
- Synonyms
- ptpra Protein, HPTPE Protein, PTPE Protein, R-PTP-EPSILON Protein, PTPe Protein, PTPepsilon Protein, RPTPepsilon Protein, protein tyrosine phosphatase, receptor type E L homeolog Protein, protein tyrosine phosphatase, receptor type E Protein, protein tyrosine phosphatase, receptor type, E Protein, ptpre.L Protein, PTPRE Protein, Ptpre Protein
- Background
- Isoform 1 acts as a negative regulator of insulin receptor (IR) signaling and is involved in insulin-induced glucose metabolism mainly through direct dephosphorylation and inactivation of IR in hepatocytes and liver (By similarity). Plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function. {ECO:0000250, ECO:0000269|PubMed:19508371, ECO:0000269|PubMed:8610169}., Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake., Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+).
- Molecular Weight
- 74.4 kDa Including tag.
- UniProt
- P49446
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