Nephronophthisis 4 (NPHP4) (AA 1-1425) protein (His tag)

Details for Product No. ABIN3134553, Supplier: Log in to see
Protein Name
  • NPHP4
  • POC10
  • SLSN4
  • 4930564O18Rik
  • nmf192
  • nephrocystin 4
  • nephrocystin-4
  • nephronophthisis 4 (juvenile) homolog (human)
  • NPHP4
  • LOC100184720
  • Nphp4
Protein Characteristics
AA 1-1425
1
1
1
1
1
Origin
Mouse (Murine)
3
Source
Insect Cells
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This NPHP4 protein is labelled with His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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Sequence MGDWHRAFTQ NTLVPPHPQR ARQLGKESTA FQCILKWLDG PLIKQGILDM LSELECHLRV TLFDVTYKHF FGRTWKTTVK PTNQPSKQPP RITFNEPLYF HTTLSHPSIV AVVEVVTEGR KRDGTLQLLS CGFGILRIFG NKPESPTSAA QDKRLRLYHG TPRALLHPLL QDPIEQNKFM RLMENCSLQY TLKPHPPLEP AFHLLPENLL VSGFQQIPGL LPPHGDTGDA LRKPRFQKPT TWHLDDLFFT LYPSLEKFEE ELVQLLISDR EGVGLLDSGT LEVLERRLHV CVHNGLGFVQ RPQVVVLVPE MDVALTRSAS FSRKISASSK NSSGNQALVL RSHLRLPEMV SHPAFAIVFQ LEYVFNSPSG ADGGASSPTS ISSVACMHMV RWAVWNPDLE VGPGKVTLPL QGGVQQNPSR CLVYKVPSAS MSSEEVKQVE SGTIQFQFSL SSDGPTEHAN GPRVGRRSSR KMPASPSGTP APAARDLAAT QDSPVGPGLS LSQLTASPLS PALQSSSKPP LQPPDSSQSP EGPQLQAESV LESRVSHLEA DLSQPASLQG TPAVEHLQEL PFTPLHAPIV VGAQTRSSRS QLSRAAMVLL QSSGFPEILD ASQQPVEAVN PIDPVRFNPQ KEESDCLRGN EIVLQFLAFS RAAQDCPGTP WPQTVYFTFQ FYRFPPETTP RLQLVKLDGT GKSGSGSLSH ILVPINKDGS FDAGSPGLQL RYMVDPGFLK PGEQRWFAHY LAAQTLQVDV WDGDSLLLIG SAGVQMKHLL RQGRPAVQVS HELEVVATEY EQEMMAVSGD VAGFGSVKPI GVHTVVKGRL HLTLANVGHA CEPRARGSNL LPPSRSRVIS NDGASFFSGG SLLIPGGPKR KRVVQAQRLA DVDSELAAML LTHTRAGQGP QAAGQEADAV HKRKLERMRL VRLQEAGGDS DSRRISLLAQ HSVRAQHSRD LQVIDAYRER TKAESIAGVL SQAITTHHTL YATLGTAEFF EFALKNPHNT QHTVAIEIDS PELSIILDSQ EWRYFKEATG LHTPLEEDMF HLRGSLAPQL YLRPRETAHI PLKFQSFSVG PLAPTQAPAE VITEKDAESG PLWKCSAMPT KHAKVLFRVE TGQLIAVLCL TVEPQPHVVD QVFRFYHPEL TFLKKAIRLP PWHTLPGAPV GMPGEDPPVH VRCSDPNVIC EAQNVGPGEP RDVFLKVASG PSPEIKDFFV VIYADRWLAV PVQTWQVCLH SLQRVDVSCV AGQLTRLSLV LRGTQTVRKV RAFTSHPQEL KTDPAGVFVL PPHGVQDLHV GVRPRRAGSR FVHLNLVDID YHQLVASWLV CLSCRQPLIS KAFEITMAAG DEKGTNKRIT YTNPYPSRRT YRLHSDRPEL LRFKEDSFQV AGGETYTIGL RFLPSGSAGQ EEILIYINDH EDKNEETFCV KVLYQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Nphp4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin free.
Grade Crystallography grade
Protein Name
Background Involved in the organization of apical junctions in kidney cells, together with NPHP1 and RPGRIP1L/NPHP8. Does not seem to be strictly required for ciliogenesis. {ECO:0000269|PubMed:21565611}.
Molecular Weight 158.2 kDa Including tag.
UniProt P59240
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Nephronophthisis 4 (NPHP4) (AA 1-1425) protein (His tag) (ABIN3134553) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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