EPH Receptor A4 Protein (EPHA4) (AA 570-986) (His tag)

Catalog No. ABIN3134799

Product Details

Target: EPH Receptor A4 (EPHA4)

Protein Type: Recombinant

Protein Characteristics: AA 570-986

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This EPH Receptor A4 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: SRRRSKYSKA KQEADEEKHL NQGVRTYVDP FTYEDPNQAV REFAKEIDAS CIKIEKVIGV GEFGEVCSGR LKVPGKREIC VAIKTLKAGY TDKQRRDFLS EASIMGQFDH PNIIHLEGVV TKCKPVMIIT EYMENGSLDA FLRKNDGRFT VIQLVGMLRG IGSGMKYLSD MSYVHRDLAA RNILVNSNLV CKVSDFGMSR VLEDDPEAAY TTRGGKIPIR WTAPEAIAYR KFTSASDVWS YGIVMWEVMS YGERPYWDMS NQDVIKAIEE GYRLPPPMDC PIALHQLMLD CWQKERSDRP KFGQIVNMLD KLIRNPNSLK RTGSESSRPN TALLDPSSPE FSAVVSVGDW LQAIKMDRYK DNFTAAGYTT LEAVVHMSQD DLARIGITAI THQNKILSSV QAMRTQMQQM HGRMVPV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Epha4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EPH Receptor A4 (EPHA4)

Alternative Name: Epha4 (EPHA4 Products)

Synonyms: HEK8 Protein, SEK Protein, TYRO1 Protein, RGD1560587 Protein, ephe Protein, EPHA4 Protein, Ci-Eph1 Protein, sek Protein, hek8 Protein, rtk1 Protein, tyr01 Protein, 2900005C20Rik Protein, AI385584 Protein, Cek8 Protein, Hek8 Protein, Sek Protein, Sek1 Protein, Tyro1 Protein, rb Protein, XEpha4 Protein, sek-1 Protein, epha4 Protein, pag Protein, EphA4 Protein, apha3 Protein, ek2 Protein, epha3 Protein, sb:cb246 Protein, zek2 Protein, zgc:109882 Protein, EPH receptor A4 Protein, Eph receptor A4 Protein, ephrin receptor epsilon Protein, EPH receptor A4 S homeolog Protein, EPH receptor A4 L homeolog Protein, eph receptor A4a Protein, EPHA4 Protein, Epha4 Protein, ephe Protein, epha4 Protein, epha4.S Protein, epha4.L Protein, epha4a Protein

Background: Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. {ECO:0000269|PubMed:15537875, ECO:0000269|PubMed:16802330, ECO:0000269|PubMed:16818734, ECO:0000269|PubMed:17143272, ECO:0000269|PubMed:17719550, ECO:0000269|PubMed:17785183, ECO:0000269|PubMed:18094260, ECO:0000269|PubMed:18403711, ECO:0000269|PubMed:9789074}.

Molecular Weight: 48.1 kDa Including tag.

UniProt: Q03137

Pathways: RTK Signaling