RIKEN cDNA 2010015L04 Gene (2010015L04RIK) (AA 1-1578) protein (Strep Tag)

Catalog No. ABIN3135110

Product Details

Target: RIKEN cDNA 2010015L04 Gene (2010015L04RIK)

Protein Type: Recombinant

Protein Characteristics: AA 1-1578

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: Strep Tag

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MEEPTVQFSD EDLVDNFPPM DDERVQLEDL EFEVERPSEG LEDEGSHSSA KKESKGAEKM RKSTTKDQVQ AFHLRKSLNL LDKMHEEKDV FIQKTKGELH ICRQRMDLLN KQQESLAAEI ATEKEANNMA AIGRLQAASR RLQTELENEK DLQSKITAML KDSENAMWHI EIQKGQFEDV RKHHEAEAEA RQRGLEVHSA RQLQREREAM EKSEKNRLLR ARKSLHTQKE LGLRHQKLVE DAQRNHRIAV KFLKASLGRV REREQKEEME SRTHMQRRMD AVLSLKNNIT ASRETLKKFQ AWGQTRADLA KQKALTEKEV ILSQGGDAFK YLFHQRRHQE LEAQKRAFEE EQKLRKQEIV NRILKEEAEE EQRKRRQHPL SKPINRRTLR DKTWQYISDF CEGKSVLTSQ QERERELLLY PKTVCVIKAI SSESVQVDLG SISTEDEVLA EPDISGLWNK ESYQVPKEDM ERKPVGGSKM EKDILARTME QLRSGVVQKQ VVSGREFKGR PFNSKPEVIH FKDFDIGKVY KKKITLINAT YTINYCKLVG VEENLKDFIH IDFEPPGPMS AGMSCEVLVT FKPMINKDLE GNVSFLAQTG SFSVPLKCST KKCSLSLDKE LIDFGTYVVG ETTSRIITLT NVGGLGTKFK FLPDSEFYEM DESQPAMKIS SLFTCEEKII YEKIMTSLSE QQLEVNDSSL VDLQSLKESE KQLDDPEVTT AAVSAMTMIP SEEQAEITLG EVTEGEIGPF SSVKVPITFT PVIPGEVQTK FKVMFKNPQS PPLYFRATGT AIDVPVWVPK ATVDLKICMY DRLYQDSITV HTRSKAALRL KFEVCKELRG HIELLPETGY IQAQSTYSVQ LKFLPRQSLP EDARKYFDPA SRVLEAPMTI RVADQIKPVR FTVQAIVTTS DLEINPSEIN FGYCTIYEAI RTEICLSNLS LLPQEFGFVG LPKYVDIQPN DGFGTILPLE TLHLDVIFQP IKAKEYKFEL VCKSEINRCF KVSCQAVGVH PPLELSHYQI KFSATSLYDT SVSTLYVINS HLSMNKMIHS LPRIGSEEAA PVGPTSFEFL LPPNSPITIS PSVGTVLPGK RCLIQVAFQP VLPKEIIYKE ASQILNKEIE TKPVSQKEIV QRKELWKQSF SVVRVHNRDR PTRVSTPQAT ELQRPVINSS STEFQIAQAT LSKAFQGKFN RFVIPCVVAS GDIKDRKTAE PLSFSPHNTL YLELWCPAVA PFIVVTSHKG KTDFNFGDIA VGHRSVKKIT LQNICNEDLT LEYSVLNPNG PFVRLNPFNK LRSGETQTLV LSFSPHENIL AQETLDIITK RGTLSLTLFG MGVASMITCS IDGNILNMGY VLARESVSTN FKLQNESSLP IKFWVRLESL SRKKAEAHQQ LPKFITSHEQ RAEIVGTQNY NGQSVFSIVP VEGLMFPGKA QEFTVTFSPD HESLFFSDLL KVVLFEKKVS HQILLKGAAR EHMMFVEGGD PLDVPVESLA VVTAFDTEHK EEAEELKPIL VTLNYVQLDT DTTTSPATRE LQVGCIRTTQ PSPRRPDHPL MVSTLLQLRG DVKETYKVTF VAHVVTGL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RIKEN cDNA 2010015L04 Gene (2010015L04RIK)

Alternative Name: Cfap74

Synonyms: A530045M11 Protein, cilia and flagella associated protein 74 Protein, Cfap74 Protein

Background: Cilia- and flagella-associated protein 74,FUNCTION: As part of the central apparatus of the cilium axoneme may play a role in cilium movement. May play an important role in sperm architecture and function. {ECO:0000250|UniProtKB:D4P3R7, ECO:0000250|UniProtKB:Q9C0B2}.

Molecular Weight: 178.9 kDa

UniProt: Q3UY96