Tenascin N Protein (TNN) (AA 27-1560) (His tag)
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- Target See all Tenascin N (TNN) products
- Tenascin N (TNN)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-1560
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This Tenascin N protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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TPESPGCSNK EQQVTVSHTY KIDVPKSALV QVETDPQSLS DDGTSLLAPG EDGEEQNIIF RHNIRLQTPQ KNCDLADSVQ DLLARMKKLE EEMAELKEQC NTNRCCQGAA DLSRHCSGHG TFLPETCSCH CDQGWEGADC DQPTCPGACN GHGRCVDGQC VCDAPYVGVD CAYAACPQDC SGHGVCVQGV CQCHEDFTAE DCSEQRCPGD CSGNGFCDTG ECYCEMGFTG PDCSQVVAPQ GLQLLKSTEN SLLVSWEPSS EVDYYLLSYY PLGKEQATKQ VRVPKEQHTY DITGLLPGTK YIVTLRNVKK DISSSPQHLL ATTDLAVVGT AWVNEETETS LDVEWENPLT EVDYYKLRYG PLTGQEVTEV TVPKSRDPKS RYDITGLQPG TEYKITVVPI RGDLEGKPIL LNGRTEIDGP TNVVTNQVTE DTASVSWDPV RADIDKYVVR YIAPDGETKE KAVPKDQSST VLTGLKPGEA YKVFVWAERG NQGSKKADTK ALTEIDSPEN LVTDRVTENS LSVSWDPVEA DIDRYVVSYT SVDGETKQVP VKKDQRSTVL TGLSPGVEYK VYVWAEKGDR ESKKANTKAP TDIDSPKNLV TDQVTENTLS VSWDPVQANI DRYMVSYTSA DGETREVPVP KEKSSTVLTG LRPGVEYKVH VWAQKGTQES RKANTKAPTD IDGPKNLVTD QVTETTLSVS WDPVEADIDR YMVRYTSPDG ETKEVPVSKD KSSTVLRGLR PGVEYKVDVW AQKGAQDSRK ANTKAPTDID SPKNLVTEQV TESTATVSWD PVEADIDRYV VRYTSVDGET REFLVGKDQT STVLTGMRPG VEYQVDVWAQ KGTQESRKTS TKAPTDIDGP KNLVTDQVTE TTLSVSWDPV EADIDRYMVR YTSPDGETKE VPVSKDKSST VLRGLRPGVE YKVDVWAQKG AQDSRKANTK APTDIDSPKN LAIDQVTETT LSVSWDPVQA DIDRYVVRYT SADGESKEFL IGKEQRSTVL TGLRPGVEYK VEVWAQKGAR ESKKANTEGH TDIDSPKNLV TNQVTENTAT ISWDPVQADI DRYMVRYTSA DGETREIPVR KEKSSTVLTG LRPGVEYTVQ VWAQKGARES KKAKTKAPTE IDSPKNLVTN RVTENTATIS WDPVRANIDR YMVRYTSADG ETKEIPVSKD QSSTILTGLK PGMEYTIHVW AQKGARESKK ADTKALTEID PPRNLRPFGV THSGGVLTWL PPSAQIDGYI LTYQFPNGTV KEVELPRGQQ RFELQDLEQG VTYPVSLVAF KGNQRSRTVS TTLSTVDARF PHPSDCSQVQ QNTNAASGLY TIYLNGDASR PMQVYCDMDT DGGGWIVFQR RNTGQLDFFK RWRSYVEGFG DPMKEFWLGL DKLHNLTTGT TTRYEVRADL QTFNESAYAV YDFFQVASSK ERYKLSVGKY RGTAGDALTY HNGWKFTTFD RDNDIALSNC ALTHHGGWWY KNCHLANPNG KYGETKHSEG VNWEPWKGHE FSIPYVELKI RPFGYSRDRF SGRKKRSIGK ARMF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Tnn Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Tenascin N (TNN)
- Alternative Name
- Tnn (TNN Products)
- Synonyms
- TNW Protein, tenascin-N Protein, TNN Protein, TN-W Protein, Tnw Protein, tenascin-W Protein, tenascin N Protein, Tnn Protein, TNN Protein
- Background
- Isoform 2 inhibits neurite outgrowth and cell migration in hippocampal explants while isoform 1 does not have this affect. {ECO:0000269|PubMed:12812753}.
- Molecular Weight
- 171.4 kDa Including tag.
- UniProt
- Q80Z71
- Pathways
- ACE Inhibitor Pathway
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