SMARCAL1 Protein (AA 2-910) (His tag)
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- Target See all SMARCAL1 Proteins
- SMARCAL1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A-Like 1 (SMARCAL1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-910
- Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This SMARCAL1 protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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SLPLTEEQRK KIEENRQKAL ARRAEKLSEQ PQSAASGSSA AGPSQSKQGS LLNLLAEPSK PVGHASIFKQ QNLSNSFPTD QRPHSSRCSQ PSPAEETTGL WKTQGEMSTA CPKPNPSPPG ASNQPLLGYK SSEGQPQATW DTGASSSGPF PRDPELEAKA ARPSTSRQSI SDSFYVLGGK TPRTEGRPPN ILQTTPQNTG FLRGACIKTG DRFRVKIGYN QELIAVFKSL PSRHYDSFTK TWDFSMSDYR ALMKAVERLS TVSLKPLDEA GGSVGGQTSL PSAPSLTFVT GKCMLISRVR FEVDIGYSEA VIGLFKQMES RSYDIKTRKW SFLLEEHNKL IARSRELKQV QLDPLPKTVT LAFASQLEKT SPKLKADVPE ADLSGVDAKL VSSLMPFQRE GVSFAISKRG RLLLADDMGL GKTVQAICIA AFYRKEWPLL VVVPSSVRFT WEQAFLRWLP SLSPENINVV VTGKGRLTAG LVNIVSFDLL CKLERQLKTP FKVVIIDESH FLKNIKTARC RAAVPILKVA KRVILLSGTP AMSRPAELYT QIIAVKPTFF PQFHAFGLRY CDAKRLPWGW DYSGSSNLGE LKLLLEEAIM LRRLKSDVLS QLPAKQRKMV VVNPGRISSR AKAALDAAAK EMTKDKTKQQ QKEALLVFFN RTAEAKIPCV VEYILDLLDS GREKFLVFAH HKVILDAVAK ELERKNVQHI RIDGSTPSAD REAQCQRFQL SKGHTVALLS ITAANMGLTF STADLVVFAE LFWNPGVLIQ AEDRVHRIGQ TNSVSIHYLV AKGTADDYLW PLIQEKIKVL GEAGLSETNF SEMTEATDYV HKDPKQKTIY DLFQQSFEDD GNDMEFLEAA ESFELGSTSG TSGNISQDLG DLLDEDEGSP PKKSRFEFFD NWDSFSSPF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Smarcal1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product SMARCAL1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SMARCAL1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A-Like 1 (SMARCAL1))
- Alternative Name
- Smarcal1 (SMARCAL1 Products)
- Synonyms
- SMARCAL1 Protein, im:7145331 Protein, si:ch211-239d11.1 Protein, harp Protein, hharp Protein, 6030401P21Rik Protein, AI851716 Protein, Mharp Protein, HARP Protein, HHARP Protein, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a like 1 Protein, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 Protein, SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 Protein, Swi/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 Protein, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 L homeolog Protein, SMARCAL1 Protein, smarcal1 Protein, Smarcal1 Protein, smarcal1.L Protein
- Background
- ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity). {ECO:0000250}.
- Molecular Weight
- 101.7 kDa Including tag.
- UniProt
- Q8BJL0
- Pathways
- Embryonic Body Morphogenesis
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