LATS1 Protein (AA 1-1129) (Strep Tag)

Catalog No. ABIN3136321

Product Details

Target: LATS1 (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1129

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This LATS1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MKRGEKPEGY RQMRPKTFPA SNYPGSSRQM LQEIRESLRN LSKPSDASKA EHNLNKMSTE DPRQVRNPPK FGTHHKALQE IRNSLLPFAN ETSSSRSPSE VNPQMFQDLQ AAGFDEDMVI QALQKTNNRS IEAAVEFISK MSYQDPRREQ MSAAAARPIN ATMKPGNVQH SINRKQSWKG SKESLVPQRH GPSLGENVVY RSESPNSQAD VGRPLSGSGI AAFAQAHPSN GQRVNPPPPP QVRSVTPPPP PRGQTPPPRG TTPPPPSWEP SSQTKRYSGN MEYVISRISP VPPGAWQEGY PPPPLTTSPM NPPSQAQRAI SSVPVGRQPI IMQSTSKFNF TPGRPGVQNG GGQSDFIVHQ NVPTGSVTRQ PPPPYPLTPA NGQSPSALQT GASAAPPSFA NGNVPQSMMV PNRNSHNMEL YNINVPGLQT AWPQSSSAPA QSSPSGGHEI PTWQPNIPVR SNSFNNPLGS RASHSANSQP SATTVTAITP APIQQPVKSM RVLKPELQTA LAPTHPSWMP QPVQTVQPTP FSEGTASSVP VIPPVAEAPS YQGPPPPYPK HLLHQNPSVP PYESVSKPCK DEQPSLPKED DSEKSADSGD SGDKEKKQIT TSPITVRKNK KDEERRESRI QSYSPQAFKF FMEQHVENVL KSHQQRLHRK KQLENEMMRV GLSQDAQDQM RKMLCQKESN YIRLKRAKMD KSMFVKIKTL GIGAFGEVCL ARKVDTKALY ATKTLRKKDV LLRNQVAHVK AERDILAEAD NEWVVRLYYS FQDKDNLYFV MDYIPGGDMM SLLIRMGIFP ENLARFYIAE LTCAVESVHK MGFIHRDIKP DNILIDRDGH IKLTDFGLCT GFRWTHDSKY YQSGDHPRQD SMDFSNEWGD PSNCRCGDRL KPLERRAARQ HQRCLAHSLV GTPNYIAPEV LLRTGYTQLC DWWSVGVILF EMLVGQPPFL AQTPLETQMK VINWQTSLHI PPQAKLSPEA SDLIIKLCRG PEDRLGKNGA DEIKAHPFFK TIDFSSDLRQ QSASYIPKIT HPTDTSNFDP VDPDKLWSDG SEEENISDTL NGWYKNGKHP EHAFYEFTFR RFFDDNGYPY NYPKPIEYEY IHSQGSEQQS DEDDQHTSSD GNNRDLVYV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: LATS1 (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1))

Alternative Name: Lats1 (LATS1 Products)

Synonyms: WARTS Protein, wts Protein, AW208599 Protein, Lats Protein, si:dkey-181i3.5 Protein, wu:fc19a11 Protein, zgc:123219 Protein, lats1 Protein, RGD1564085 Protein, warts Protein, large tumor suppressor kinase 1 Protein, large tumor suppressor Protein, large tumor suppressor kinase 1 S homeolog Protein, LATS1 Protein, Lats1 Protein, lats1 Protein, lats1.S Protein

Background: Serine/threonine-protein kinase LATS1 (EC 2.7.11.1) (Large tumor suppressor homolog 1) (WARTS protein kinase),FUNCTION: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:21145499). Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cell differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1. {ECO:0000250|UniProtKB:O95835, ECO:0000269|PubMed:21145499, ECO:0000269|PubMed:9988269}.

Molecular Weight: 126.3 kDa

UniProt: Q8BYR2

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location