MLK4 Protein (AA 1-1002) (Strep Tag)

Catalog No. ABIN3136719

Product Details

Target: MLK4 (KIAA1804) (Mitogen-Activated Protein Kinase Kinase Kinase MLK4 (KIAA1804))

Protein Type: Recombinant

Protein Characteristics: AA 1-1002

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MLK4 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MALPVAEGTA DTPLSPARDD SGSTSSGMWA ALYDYEARGE DELSLRRGQL VEVLSQDAAV SGDEGWWAGQ VQRRLGIFPA SYVAPCGPVP PPAPPPPRPC SPVHVDFERL ELKELIGAGG FGQVYRATWQ GQEVAVKAAR RDPEQDAAAA AESVRREARL FAMLRHPNII QLRGVCLRQP HLCLVLEFAR GGALNRALAA AASDPRAPGP RRARRIPPQV LVNWAVQIAR GMLYLHEEAV VPILHRDLKS SNILLLEKIE HDDICNKTLK ITDFGLAREW HRTTRMSAAG TYAWMAPEVI RSSLFSKGSD IWSYGVLLWE LLTGEVPYRG IDGLAVAYGV AVNKLTLPIP STCPEPFAKL MKECWEQDPH IRPSFALILQ QLTAIEEAVL TNMPQESFHS MQEDWKLEIQ QMFSELRTKE KELRSREEEL SRAALQQKSQ ELLLRRREQQ LAEREIDVLE RELNVLIFQL SQEAPHVKKR KGRFRRGRLR LKDGHRISLP SDFQHKITVQ ASPTLDKRRS SDSGLCSPPG SPLMLPRLRA IQLTSDENNK TRGRNMVFRQ EDFEDVKRSF KKKGCTWGPS SVQTKERPEG RERVRPLSDG NSPWSSLLIK SQKTTPLASL FVDQPGSCEE QKLVPEGLEH RKPKQTKFPG QAHVGLPLCK DSQREDSSEA ESREEGSPKG SPVNNVGAPM LRKKTESALC ECGMLLASMA LGLDVRKLHG AQAPAKPSPK MEKKEEGALQ PASRCQSSPS SLLRQPSAGR APSGGSTLLL PSAPSHSSKS SLSMKCLLQA GKEESSLGNA RDLCGPTTLT PDPGSAAPES GCELIPGLRP KTDYGVLRSM PHAILEQTGE RLPGCAIVGD KGCHHMQMGS EETPLWLQSA PEDSGLPHSP SPGPQRDLAS QASLVKPEGV LGECQACPAL PQRPHTASVR TTSPPTWVCD KDHQVPALAC LLGAQERSRC QTPSLLDASI EGQKKDCAMP LCRVKSVMCQ PSIYALEKDF LT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MLK4 (KIAA1804) (Mitogen-Activated Protein Kinase Kinase Kinase MLK4 (KIAA1804))

Alternative Name: Map3k21 (KIAA1804 Products)

Synonyms: MLK4 Protein, RP5-862P8.2 Protein, dJ862P8.3 Protein, Mlk4 Protein, mKIAA1804 Protein, mitogen-activated protein kinase kinase kinase 21 Protein, MAP3K21 Protein, Map3k21 Protein

Background: Mitogen-activated protein kinase kinase kinase 21 (EC 2.7.11.25) (Mitogen-activated protein kinase kinase kinase MLK4) (Mixed lineage kinase 4),FUNCTION: Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways (By similarity). {ECO:0000250}.

Molecular Weight: 110.1 kDa

UniProt: Q8VDG6