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EXD2 Protein (AA 1-650) (Strep Tag)

EXD2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, SDS, WB
Catalog No. ABIN3136735
  • Target See all EXD2 products
    EXD2 (Exonuclease 3'-5' Domain Containing 2 (EXD2))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-650
    Origin
    Mouse
    Source
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This EXD2 protein is labelled with Strep Tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MSRQNLVALT VTTLLGVAMG GFVLWKGIQR RWSKTSRVMQ QQPQQPQQPQ QPQPQPQPQP QPQPEHPQPQ QQVPGGREWP PPEDDQLPFG ALRAPRASWE ERILQAEVVT VSQEAEWNQI QPFLKRELED FPVLGIDCEW VNLEGKASPL SLLQMASPSG FCALVRLPRL IYGGRTLPRT LLDILADGAI LKVGVGCSED ANKLLQDYGL IVRGCLDLRY LAMKQGNNIL CNGLSLKSLA ETILNFPLDK SLLLRCSNWD AENLTEDQVT YAARDAQISV ALFLHLLGYP FSRDSYEEES TDQINWQKAL ERCRNMVDIP FRSKGLGRLV EEVNGEALES QLKPRNRKAK TDRMVPGNNQ GRDPRKHKRK PLGVGYSARK SPLYDNCFLQ APDGQPLCTC DRRKAQWYLD KGIGELVSKE PFVVRLQFEP AGRPESPGDY YLMVKENLCV VCGKTDTYIR KNIIPHEYRK HFPIEMKDHN SHDVLLLCTS CHAISNYYDN HLKQQLAKEF QAPIGSEEGL RLLEDLERRQ VRSGARALLN AESLPAHRKE ELLHALREFY NTDIITEEML HEAASLETRI YNESYIPHGL KVVQRHTEGG LRSLMQLESR WRQHFLDSMQ PKHLPQQWSV DHNHQKLLRK YGDDLPIKLS
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Purity
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    EXD2 (Exonuclease 3'-5' Domain Containing 2 (EXD2))
    Alternative Name
    Exd2 (EXD2 Products)
    Synonyms
    C14orf114 Protein, EXDL2 Protein, 4930539P14Rik Protein, C85658 Protein, Exdl2 Protein, RGD1311087 Protein, exd2-b Protein, exdl2 Protein, fi72h12 Protein, wu:fi72h12 Protein, zgc:175195 Protein, exd2 Protein, exd2-a Protein, exonuclease 3'-5' domain containing 2 Protein, exonuclease 3'-5' domain containing 2 b Protein, EXD2 Protein, Exd2 Protein, exd2.S Protein, exd2 Protein, exd2.L Protein
    Background
    Exonuclease 3'-5' domain-containing protein 2 (EC 3.1.11.1) (3'-5' exoribonuclease EXD2) (EC 3.1.13.-) (Exonuclease 3'-5' domain-like-containing protein 2),FUNCTION: Exonuclease that has both 3'-5' exoribonuclease and exodeoxyribonuclease activities, depending on the divalent metal cation used as cofactor. In presence of Mg(2+), only shows 3'-5' exoribonuclease activity, while it shows both exoribonuclease and exodeoxyribonuclease activities in presence of Mn(2+). Acts as an exoribonuclease in mitochondrion, possibly by regulating ATP production and mitochondrial translation. Also involved in the response to DNA damage. Acts as 3'-5' exodeoxyribonuclease for double-strand breaks resection and efficient homologous recombination. Plays a key role in controlling the initial steps of chromosomal break repair, it is recruited to chromatin in a damage-dependent manner and functionally interacts with the MRN complex to accelerate resection through its 3'-5' exonuclease activity, which efficiently processes double-stranded DNA substrates containing nicks. Also involved in response to replicative stress: recruited to stalled forks and is required to stabilize and restart stalled replication forks by restraining excessive fork regression, thereby suppressing their degradation. {ECO:0000250|UniProtKB:Q9NVH0}.
    Molecular Weight
    74.3 kDa
    UniProt
    Q8VEG4
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