ECD/SGT1 Protein (AA 1-641) (Strep Tag)

Catalog No. ABIN3137032

Product Details

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Protein Type: Recombinant

Protein Characteristics: AA 1-641

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ECD/SGT1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEGSGKLAMV EDAVEYHLFL IPDKARGTEE HREILQKYIE RIMTQFAPIL VPYIWQNQPF NLKYKPAKGG VPAHMYGMTK FGDNIEDEWF IVYVIKQITK EFPELVARVE DNDGEFLLIE AADFLPKWLD PDNSANRVFF HHGELCIIPV PRKSERIPWL PMTPPTIQQA LSIISAHPEA VLASESIQAA VDRRVSGYPE RVEASLHRAH CFLPAGIVAV LKQQPRLLSA AVQAFYLRDP IDLRACRVFK TFLPETRIMA SVTFTKCLYA QLVQQKFVPD RRSGYGLPPP SHPQYRAYEL GMKLAHGFEI LCSKCSPHFS DSRKSLVTAS PLWASFLESL KRNDYFKGLM DGSAQYQERL EMAKNYFQLS IHRPESSLAM SPGEEILTVL QTQPFDVAEL KTEEADLPPE DDDQWLDLSP DQLDQLLQDA AGRKESQPGP QKEELQNYDV AQVSDSMKAF ISKVSSHKGA ELPRDPSEAP ITFDADSFLN YFDKILGAKP QESDSEDDPG EEDVEGVDSD DDVGFEAQES ESLKGALGSL KSYMARMDQE LAHTSMGRSF TTRERLNKDP PSHTANDNSD EEDSGAGDCA VEAVDVDLNL ISNILESYSS QAGLAGPASN LLHSMGVRLP DNADHNPQVS Q
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Alternative Name: Ecd (ECD Products)

Synonyms: GCR2 Protein, HSGT1 Protein, 5730461K03Rik Protein, Hsgt1 Protein, ecdysoneless cell cycle regulator Protein, ecdysoneless homolog (Drosophila) Protein, ECD Protein, Ecd Protein

Background: Protein ecdysoneless homolog,FUNCTION: Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (By similarity). Involved in regulation of cell cycle progression (PubMed:26711270). Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing (By similarity). {ECO:0000250|UniProtKB:O95905}.

Molecular Weight: 71.7 kDa

UniProt: Q9CS74

Pathways: Regulation of Carbohydrate Metabolic Process