INCENP Protein (AA 1-880) (Strep Tag)

Catalog No. ABIN3137503

Product Details

Target: INCENP (Inner Centromere Protein Antigens 135/155kDa (INCENP))

Protein Type: Recombinant

Protein Characteristics: AA 1-880

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This INCENP protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: MGTTAPGPIC LLDLCDQKLL DFVCNVDNKD FMWLKEIEEE AERMFIREFS NEPELMPKTP SQKNRRKKRR VSNIQDENRD PVRKRLSRRK SRSSQVGTRH LRSKPVTIVE ENGFPVLQRI TRATAAAAAA AAAASVASAS SSSTAGSPTV LTKKAVVEIS TSERLSAELQ LTKLKGSLPP SPVSQGTLTS EEELTPKKSE AGKLDSVTVN SLKATPQSPK NRGVGEGRSV SKLKIARASW GLQDSPGSTD SPWQERVLSP ILLNNILPTT AKSPLGNIRS VRRSLISQDS QVPLASKYNL VAKQENGSRR SSRRIAKKAG KEPEASARII CHSYLERLLN VEVPQNVGLE QEPVEVAEPE EAEEEQEVSK NSGCPSKPRS ATKIAISTPT SKPAAAGQTT TVEEQEAELD QTDGHREPPQ SVRRKRSYKQ AISEPDEEQL EDEELQPCQN KTPSPPCPAN KVVRPLRTFL HTVQKNQMLM TPTLASRSSV MKSFIKRNTP LRVDPKCSFV EKERQRLESL RRKEEAEQRR RQKVEEDKRR RLEEVKLKRE ERLRKVLQAR ERVEQMKEEK KKQIEQKFAQ IDEKTEKAKE ERLAEKAKKK ATAKKMEEVE ARRKQEEEAR RLRWLQQEEE ERRHQEMLQR KKEEEQERRK AAEARRLAEQ REQERRREQE RREQERREQE RREQERKEQE RREQEQERLR AKREMQEREK ALRLQKERLQ KELEEKKRKE EQQRLAEQQL QEEQAKKAKE VAAARKVLNM TVDVQSPVCT SYQMTPQGPK SIPKISVDDY GMDLNSDDST DDESHPRKPI PSWAKGTQLS QAIVHQYYHP PNILELFGSI LPLDLEDIFK KRKTRYHKRT SSAVWNSPPL KATMVPSSGD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: INCENP (Inner Centromere Protein Antigens 135/155kDa (INCENP))

Alternative Name: Incenp (INCENP Products)

Synonyms: INCENP Protein, LOC572121 Protein, 2700067E22Rik Protein, AU019509 Protein, C130081E20 Protein, C77457 Protein, XL-INCENP Protein, incenp Protein, incenp-a Protein, incenp-b Protein, xINC Protein, xIncenp Protein, xl-incenp Protein, inner centromere protein Protein, inner centromere protein S homeolog Protein, inner centromere protein L homeolog Protein, INCENP Protein, Incenp Protein, incenp.S Protein, incenp Protein, incenp.L Protein

Background: Inner centromere protein,FUNCTION: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules. The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity). Activates AURKB and AURKC. Controls the kinetochore localization of BUB1. {ECO:0000250|UniProtKB:P53352, ECO:0000250|UniProtKB:Q9NQS7}.

Molecular Weight: 101.2 kDa

UniProt: Q9WU62

Pathways: Cell Division Cycle