Regulator of G Protein Signaling 9 Binding Protein (RGS9BP) (AA 1-237) protein (Strep Tag)

Catalog No. ABIN3121603

Product Details

Target: Regulator of G Protein Signaling 9 Binding Protein (RGS9BP)

Protein Type: Recombinant

Protein Characteristics: AA 1-237

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: Strep Tag

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MAREECKALL DALNKTTACY HHLVLTVGGS ADTQDLREEL QKTRQKAREL AVATGARLTV ALRDRSLATE ERAEFERLWV AFSGCLDLLE ADMQRALALG ATFPLHAPRR PLVRTGVTGG SSAVAARALS ARSLRHEAES DFDVADLPQL EREVLQVGEM IDDMEMKVNV PRWTVQARQA AGAELLSGAS AGASSAGGIS VEERAGPCDP SKALAATVFS AVLLVAVALA LCVAKLS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Regulator of G Protein Signaling 9 Binding Protein (RGS9BP)

Alternative Name: Rgs9bp (RGS9BP Products)

Synonyms: PERRS Protein, R9AP Protein, RGS9 Protein, perrs Protein, r9ap Protein, rgs9 Protein, rgs9bp-a Protein, Rgs9-1bp Protein, rgs9bp Protein, regulator of G protein signaling 9 binding protein Protein, regulator of G-protein signaling 9 binding protein Protein, regulator of G-protein signaling 9 binding protein S homeolog Protein, regulator of G-protein signalling 9 binding protein Protein, zgc:109913 Protein, RGS9BP Protein, rgs9bp.S Protein, Rgs9bp Protein, zgc:109913 Protein

Background: Regulator of G-protein signaling 9-binding protein (RGS9-anchoring protein),FUNCTION: Regulator of G protein-coupled receptor (GPCR) signaling in phototransduction. Participates in the recovery phase of visual transduction via its interaction with RGS9-1 isoform. Acts as a membrane-anchor that mediates the targeting of RGS9-1 to the photoreceptor outer segment, where phototransduction takes place. Enhances the ability of RGS9-1 to stimulate G protein GTPase activity, allowing the visual signal to be terminated on the physiologically time scale. It also controls the proteolytic stability of RGS9-1, probably by protecting it from degradation. {ECO:0000269|PubMed:12560335, ECO:0000269|PubMed:14625292, ECO:0000269|PubMed:16908407, ECO:0000269|PubMed:16939221}.

Molecular Weight: 25.2 kDa

UniProt: Q148R9

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling