PPM1L Protein (AA 43-360) (His tag)
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- Target See all PPM1L Proteins
- PPM1L (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1L (PPM1L))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 43-360
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PPM1L protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- Sequence
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DEVKTIVKSS RDAVKMVKGK VAEIMQNDRL GGLDVLEAEF SKTWEFKSHN VAVYSIQGRR DHMEDRFEVL TDLANKTHPS IFGIFDGHGG ETAAEYVKSR LPEALKQHLQ DYEKDKENSV LTYQTILEQQ ILSIDREMLE KLTVSYDEAG TTCLIALLSD KDLTVANVGD SRGVLCDKDG NAIPLSHDHK PYQLKERKRI KRAGGFISFN GSWRVQGILA MSRSLGDYPL KNLNVVIPDP DILTFDLDKL QPEFMILASD GLWDAFSNEE AVRFIKERLD EPHFGAKSIV LQSFYRGCPD NITVMVVKFR NSSKTEEH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Ppm1l Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Grade
- Crystallography grade
- Top Product
- Discover our top product PPM1L Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- PPM1L (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1L (PPM1L))
- Alternative Name
- Ppm1l (PPM1L Products)
- Synonyms
- 3222401G13 Protein, 5930404J21Rik Protein, AI481720 Protein, AW045850 Protein, PP2C Protein, PP2Cepsilon Protein, Pp2ce Protein, Ppp2ce Protein, mKIAA4175 Protein, PP2C-epsilon Protein, PP2CE Protein, PPM1-LIKE Protein, ppm1l Protein, zgc:153235 Protein, zgc:154091 Protein, protein phosphatase 1 (formerly 2C)-like Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1L Protein, protein phosphatase, Mg2+/Mn2+ dependent 1L Protein, protein phosphatase, Mg2+/Mn2+ dependent 1L L homeolog Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1Lb Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1La Protein, Ppm1l Protein, PPM1L Protein, ppm1l.L Protein, ppm1lb Protein, ppm1la Protein
- Background
- Acts as a suppressor of the SAPK signaling pathways by associating with and dephosphorylating MAP3K7/TAK1 and MAP3K5, and by attenuating the association between MAP3K7/TAK1 and MAP2K4 or MAP2K6. {ECO:0000269|PubMed:12556533}.
- Molecular Weight
- 37.0 kDa Including tag.
- UniProt
- Q8BHN0
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