ANKAR Protein (AA 1-1465) (Strep Tag)
Crystallography grade
ANKAR
Origin: Mouse
Host: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB
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- Target See all ANKAR products
- ANKAR (Ankyrin and Armadillo Repeat Containing (ANKAR))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1465
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This ANKAR protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MSRIVKKGIV KIDQEQDEET FRENLAVQRN ASAFFEKYDR TEVQELLTTT LVSWLAAKDD ARSQLETPCG LMSQMNNAGF STAILLTPVD PTALLDYREV HQILRELAIG IYCLNQIPSI SLEANFDQSS SCQLPPAYYD TRVGQILIQI DYMLKALWHG IYMPKEKRAR FSELWRTIMD IDLDGKPQTT KNVFSEFSSA GLVDITNDPD FNGIYDEDMN EDPTYEPNSP EEKAVFMKYA ESIMMKLTFS TVQIQQHENI FIFETAYWLS NAIKYNQDYL DICTYQRLQK RLYLQKKVIQ KHFEKKKEIR RGMGYLKLIC FLIPFLLSLK RKMKVPYLNS LLPPFSDDKV KTERELPPFI YGRDFKCQNF DYKQHQYFHV HGGIEFDIST HPVESALDEF KKNVEKIWEC ASSASAEDAG YKEVYPIPVM ELNGKSYYVI HFELEIFYQQ LYKTQWWVAI NETVNNLKVK RLPLTEDQLH EQFKKKFGLK KAMKCKSIPF GVKSAVERGL SAVFYTFSRK TSSSTINVSD EAGYAIFHHA ALHNRVSVIC QLWSANFNVN QRRFIMFSQA DSSKVDMKKE RNGPTPLHLA AQACSLEATI CLLCFKADYT LTEKRGWMPI HFAAFYDNIC ILIALCRKDP SLLEAEATAE NQCTPLLLAA TSGALDTIQY LFSLGANWRK TDTKGNNIIH LSVLAFHTEV LKYIIELNIP ELPVWETLVE MLQCESSKRR MMAVMSLEVI CLANDRYWQC ILDAGTIPAL VNLLKSPQIK LQYKTVGLLS NISTHVSIVH AIVEAGGIPA VINLLTSDEP ELHSRCAIIL YDVAKCENKD VIAKYSGIPA LINLLSLNKE SVLVNVMNCI RVLCMGNESN QQSMKDNNGI QYLIQFLSSD SDVLKALSSA TIAEVARDNK EVQDAIAKEG AIPPLVTLFK GKQLSVQVKG AMAVESLANC NPLIQKEFLE RELTKDLLKL LQAFQIDVKE QGAIALWALA GQTLKQQKYM AEQIGYNLII SMLLSPSAKM QYVGGEAVIA LSKDSRMHQN QICEGKGIAP LVRLLRINKI PEGTLLSVIR AVGSICIGVA HTSNPMSQQF VVEENALPVL IQLLRNHPSI NIRVEVAFSL ACIVLGNNSL KKELQNDEGF EYSDVLYLLH SKDKEVCLKA GYALTLFAFN DRFQQHLILE TGLITVSIFE PFLQSSVETE RAMAAFQIII LAKAIIDVEH VTLYGRGIQI LADSLNSVHA PTIALTGNII ASLAHSRAGI PEAFVSLGTV QRLCYHLYAR SEEVRTACSC ALGYLTYNAH AFRLLLTECR NKPNQFLRIT NNISKDAKIN PAFLKEFQLQ QRMGLPSLSL ERNGGPPVIP VFKKGKEHRQ KTRPKIQPRD SLTLLPPVTN VKELFRTTHK ANISHNTFSF PSGVSSDIIN VSRPRIAFLN KLGKDEQKAN PDPPAFLNKL GKDEQNANPD PAESQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ANKAR (Ankyrin and Armadillo Repeat Containing (ANKAR))
- Alternative Name
- Ankar (ANKAR Products)
- Synonyms
- 4932422E22Rik Protein, ankyrin and armadillo repeat containing Protein, ANKAR Protein, Ankar Protein
- Background
- Ankyrin and armadillo repeat-containing protein
- Molecular Weight
- 165.3 kDa
- UniProt
- A2RT91
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