DRD4 Protein (AA 1-387) (Strep Tag)

Catalog No. ABIN3134352

Product Details

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Protein Type: Recombinant

Protein Characteristics: AA 1-387

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This DRD4 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MGNSSATEDG GLLAGRGPES LGTGAGLGGA GAAALVGGVL LIGLVLAGNS LVCVSVASER TLQTPTNYFI VSLAAADLLL AVLVLPLFVY SEVQGGVWLL SPRLCDTLMA MDVMLCTASI FNLCAISVDR FVAVTVPLRY NQQGQCQLLL IAATWLLSAA VASPVVCGLN DVPGRDPAVC CLENRDYVVY SSVCSFFLPC PLMLLLYWAT FRGLRRWEAA RHTKLHSRAP RRPSGPGPPV SDPTQGPFFP DCPPPLPSLR TSPSDSSRPE SELSQRPCSP GCLLADAALP QPPEPSSRRR RGAKITGRER KAMRVLPVVV GAFLVCWTPF FVVHITRALC PACFVSPRLV SAVTWLGYVN SALNPIIYTI FNAEFRSVFR KTLRLRC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Alternative Name: Drd4 (DRD4 Products)

Synonyms: Drd4b Protein, si:ch211-69h5.1 Protein, zgc:123021 Protein, D4DR Protein, AW125663 Protein, D4R Protein, Drd-4 Protein, D4RA Protein, DRD4 Protein, Drd4c Protein, dopamine receptor D4 related sequence Protein, dopamine receptor D4 Protein, dopamine receptor D4b Protein, drd4-rs Protein, DRD4 Protein, Drd4 Protein, SRU_2808 Protein, MSMEG_3522 Protein, drd4b Protein

Background: D(4) dopamine receptor (D(2C) dopamine receptor) (Dopamine D4 receptor),FUNCTION: Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Activated by dopamine, but also by epinephrine and norepinephrine, and by numerous synthetic agonists and drugs. Agonist binding triggers signaling via G proteins that inhibit adenylyl cyclase (By similarity). Modulates the circadian rhythm of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells (PubMed:24048828). {ECO:0000250|UniProtKB:P21917, ECO:0000269|PubMed:24048828}.

Molecular Weight: 41.5 kDa

UniProt: P51436

Pathways: cAMP Metabolic Process, Synaptic Membrane, Proton Transport, Photoperiodism, Negative Regulation of Transporter Activity