PGAP1 Protein (AA 1-922) (Strep Tag)

Catalog No. ABIN3135098

Product Details

Target: PGAP1 (Post-GPI Attachment To Proteins 1 (PGAP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-922

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PGAP1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MFLHSVNLWN LAFYVFMVFL ATLGLWDVFF GFEENKCSMS YMFEYPEYQK IELPKKLTKR YPAYELYLYG EGSYAEEHKI LPLTGIPVLF LPGNAGSYKQ VRSIGSIALR KAEDIDFKYH FDFFSVNFNE ELVALYGGSL QKQTKFVHEC IKAILKLYKG QEFAPTSVAI IGHSMGGLVA RALLTLKNFK QDLINLLVTQ ATPHVAPVMP LDRFITEFYM NVNNYWILNA RHINLTTLSV AGGFRDYQVR SGLTFLPKLS HYTSALSVVS SAVPKTWVST DHLSIVWCKQ LQLTTIRAFF DLIDADTKQI TQKPKKKLSV LNHHFIRHPA KQFEENPSII SDLTGTSMWV PVKVSRWSYV AYNESDKIYF AFPLANHRKI YTHAYCQSTM LDTNSWIFGC INSTSMCRQG VDLSWKAELL PTIKSLTLRL QDYPSLSHIV VYVPSVHGSK FVVDCEFFKK EARSMQLPVT HLFSFGLSSR KVTLNTNGLY YNIELLNFGQ IYQAFKVNVV SKCTGSKEEI TSIYKLHIPW SYEDSLTIAQ VPSSTDISLK LHVAQPENDS HVALLKMYTS SDCQYEVTIK TSFPQILGQV VRFHGGALPA YVVSSILLAY GGQLYSLLST GYCLEYSTIL DKEAKPYKVD PFVIMIKFLL GYKWFKELWD AVLLPELDAI VLTSQSMCFP LVSLILFLFG TCTAYWSGLL SSTSVQLLSS LWLALKRPAE LPKDIKVMSP DLPVLTVVFL IVSWTTCGAL AILLSYLYYV FKVVHLQASL TTFKNNQPVN PKHSRRSEKK SNHHKDSAVQ SLRLCANDAE DSLRMHSTVI NLLTWVVLLS MPSLIYWLKN LRYYFKLSPD PCKPLAFLLI PAIAILGNTH TVSVKSSKLL KTVSQFPLPL AVGVIAFGSS HLYRVPCFVI IPLVFHALCN FM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PGAP1 (Post-GPI Attachment To Proteins 1 (PGAP1))

Alternative Name: Pgap1 (PGAP1 Products)

Synonyms: Bst1 Protein, ISPD3024 Protein, 5033403E17Rik Protein, 9030223K07Rik Protein, A530084K22 Protein, D230012E17Rik Protein, oto Protein, bst1 Protein, post-GPI attachment to proteins 1 Protein, bone marrow stromal cell antigen 1 Protein, post-GPI attachment to proteins 1 L homeolog Protein, Pgap1 Protein, PGAP1 Protein, Bst1 Protein, pgap1.L Protein

Background: GPI inositol-deacylase (EC 3.1.-.-) (Post-GPI attachment to proteins factor 1),FUNCTION: Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi (By similarity). {ECO:0000250}.

Molecular Weight: 104.6 kDa

UniProt: Q3UUQ7

Pathways: Sensory Perception of Sound, Inositol Metabolic Process