Transmembrane 7 Superfamily Member 4 (TM7SF4) (AA 1-470) protein (Strep Tag)
TM7SF4
Origin: Mouse
Host: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB
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- Target See all Transmembrane 7 Superfamily Member 4 (TM7SF4) products
- Transmembrane 7 Superfamily Member 4 (TM7SF4)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-470
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- Strep Tag
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MRLWTLGTSI FLRLWGTYVF PRSPSWLDFI QHLGVCCFVA FLSVSLFSAA FYWILPPVAL LSSVWMITCV FLCCSKRARC FILLAVLSCG LREGRNALIA AGTGVVIFGH VENIFYNFRG LLDSMTCNLR AKSFSVHFPL LKRYTEAIQW IYGLATPLNL FDDLVSWNQT LVVSLFSPSH ALEAHMNDTR GEVLGVLHHM VVTTELLTSV GQKLLALAGL LLILVSTGLF LKRFLGPCGW KYENVYITKQ FVRFDEKERH QQRPCVLPLN KKERKKYVIV PSLQLTPKEK KTLGLFFLPV LTYLYMWVLF AAVDYLLYRL ISSMNKQFQS LPGLEVHLKL RGEKQGTQGV VHDSAFNISM FEPSCIPKPR LSVSETWVPL SIILLTLIIL GLLSSMLMQL KILVSVSFYP KVERERIEYL HAKLLEKRSK QPLREADGKP SLYFKKIHFW FPVLKMIRKK QTIPANEDDL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Transmembrane 7 Superfamily Member 4 (TM7SF4)
- Alternative Name
- Dcstamp (TM7SF4 Products)
- Synonyms
- Tm7sf4 Protein, TM7SF4 Protein, FIND Protein, hDC-STAMP Protein, 4833414I07Rik Protein, DC-STAMP Protein, mDC-STAMP Protein, dendrocyte expressed seven transmembrane protein Protein, Dcstamp Protein, DCSTAMP Protein
- Background
- Dendritic cell-specific transmembrane protein (DC-STAMP) (mDC-STAMP) (Dendrocyte-expressed seven transmembrane protein) (Transmembrane 7 superfamily member 4),FUNCTION: Probable cell surface receptor that plays several roles in cellular fusion, cell differentiation, bone and immune homeostasis. Plays a role in TNFSF11-mediated osteoclastogenesis. Cooperates with OCSTAMP in modulating cell-cell fusion in both osteoclasts and foreign body giant cells (FBGCs). Participates in osteoclast bone resorption. Involved in inducing the expression of tartrate-resistant acid phosphatase in osteoclast precursors. Plays a role in haematopoietic stem cell differentiation of bone marrow cells toward the myeloid lineage. Inhibits the development of neutrophilic granulocytes. Plays also a role in the regulation of dendritic cell (DC) antigen presentation activity by controlling phagocytic activity. Involved in the maintenance of immune self-tolerance and avoidance of autoimmune reactions. {ECO:0000269|PubMed:15452179, ECO:0000269|PubMed:16061724, ECO:0000269|PubMed:16937266, ECO:0000269|PubMed:17164993, ECO:0000269|PubMed:17713547, ECO:0000269|PubMed:18653699, ECO:0000269|PubMed:18952287, ECO:0000269|PubMed:20039274, ECO:0000269|PubMed:22337159}.
- Molecular Weight
- 53.9 kDa
- UniProt
- Q7TNJ0
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