KARS Protein (AA 2-597) (His tag)

Catalog No. ABIN7312722

Product Details

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Protein Type: Recombinant

Protein Characteristics: AA 2-597

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This KARS protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Mass Spectrometry (MS)

Purpose: Recombinant Human KARS Protein is produced by our mammalian expression system and the target gene encoding Ala2-Val597 is expressed with a 6His tag at the C-terminus.

Sequence: AAVQAAEVKV DGSEPKLSKN ELKRRLKAEK KVAEKEAKQK ELSEKQLSQA TAAATNHTTD NGVGPEEESV DPNQYYKIRS QAIHQLKVNG EDPYPHKFHV DISLTDFIQK YSHLQPGDHL TDITLKVAGR IHAKRASGGK LIFYDLRGEG VKLQVMANSR NYKSEEEFIH INNKLRRGDI IGVQGNPGKT KKGELSIIPY EITLLSPCLH MLPHLHFGLK DKETRYRQRY LDLILNDFVR QKFIIRSKII TYIRSFLDEL GFLEIETPMM NIIPGGAVAK PFITYHNELD MNLYMRIAPE LYHKMLVVGG IDRVYEIGRQ FRNEGIDLTH NPEFTTCEFY MAYADYHDLM EITEKMVSGM VKHITGSYKV TYHPDGPEGQ AYDVDFTPPF RRINMVEELE KALGMKLPET NLFETEETRK ILDDICVAKA VECPPPRTTA RLLDKLVGEF LEVTCINPTF ICDHPQIMSP LAKWHRSKEG LTERFELFVM KKEICNAYTE LNDPMRQRQL FEEQAKAKAA GDDEAMFIDE NFCTALEYGL PPTAGWGMGI DRVAMFLTDS NNIKEVLLFP AMKPEDKKEN VATTDTLEST TVGTSVVDHH HHHH

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 20 % glycerol, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Alternative Name: KARS (KARS Products)

Synonyms: CG12141 Protein, Dmel\\CG12141 Protein, KRS Protein, LysRS Protein, cb530 Protein, wu:fa16h02 Protein, zgc:92483 Protein, kars Protein, MGC53345 Protein, kars2 Protein, krs Protein, krs-1 Protein, KARS Protein, Tb06.28P18.190 Protein, CMTRIB Protein, DFNB89 Protein, KARS1 Protein, KARS2 Protein, AA589550 Protein, AL024334 Protein, AL033315 Protein, AL033367 Protein, D8Ertd698e Protein, D8Wsu108e Protein, mKIAA0070 Protein, Lysyl-tRNA synthetase Protein, lysyl-tRNA synthetase Protein, lysyl-tRNA synthetase S homeolog Protein, lysyl-tRNA synthetase L homeolog Protein, LysRS Protein, kars Protein, kars.S Protein, kars.L Protein, Bm1_16500 Protein, lysS Protein, Taci_1118 Protein, Tpau_1737 Protein, Arch_0261 Protein, Trad_2960 Protein, Spirs_3699 Protein, KARS Protein, LOC100280510 Protein, Tb927.6.1510 Protein, Kars Protein

Gene ID: 3735

UniProt: Q15046