IL23 Protein

Catalog No. ABIN5505699

Product Details

Target: IL23 (Interleukin 23 (IL23))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Insect cells (Sf9)

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Purpose: Purified recombinant protein of Human interleukin 23 (p19+p40).

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics: Human IL-23 consists of two subunits linked via a disulfide bond: P19, the region of Ala21- Pro189) from Accession# NP_057668, and P40, the region of Ile 23-Ser 328) from Accession# NP_002178.2

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Endotoxin Level: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 0.4 - 2.0 ng/ml, corresponding to a specific activity of 0.5 - 2.50 x 10^6 units/mg determined by mouse splenocyte IL-17A secretion, which is induced by hIL-23 in a dose dependent manner.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 1 x PBS

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: IL23 (Interleukin 23 (IL23))

Alternative Name: IL23 (IL23 Products)

Synonyms: IL-23 Protein, p19 Protein, SGRF Protein, il23p19 Protein, interleukin 23, alpha subunit p19 Protein, interleukin 23 subunit alpha Protein, Il23a Protein, IL23A Protein

Background: This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells.

Molecular Weight: 53.3 kDa

NCBI Accession: NP_057668

UniProt: Q9NPF7