UCHL3 Protein (partial) (His tag)
-
- Target See all UCHL3 (Uchl3) Proteins
- UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))
- Protein Type
- Recombinant
- Protein Characteristics
- partial
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UCHL3 protein is labelled with His tag.
- Application
- Western Blotting (WB)
- Sequence
- Glu 2 - Ala 230
- Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Endotoxin level is less than 1.0 EU per ug by the LAL method.
- Top Product
- Discover our top product Uchl3 Protein
-
-
- Application Notes
- This recombinant protein can be used for WB.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Buffer
- 50 mM Tris, 150 mM NaCl, pH 7.5
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C.
-
- Target
- UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))
- Alternative Name
- UCH-L3 (Uchl3 Products)
- Synonyms
- UCH-L3 Protein, UCH-6 Protein, RGD1561196 Protein, im:6908825 Protein, zgc:109963 Protein, uchl4 Protein, ubiquitin C-terminal hydrolase L3 Protein, ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) Protein, ubiquitin C-terminal hydrolase L3 L homeolog Protein, UCHL3 Protein, Uchl3 Protein, uchl3 Protein, uchl3.L Protein
- Background
- Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCH-L3), a member of peptidase C12 family, is also known as ubiquitin thioesterase L3. Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. UCH-L3 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is also required for stress-response retinal, skeletal muscle and germ cell maintenance. Furthermore, UCH-L3 may be involved in working memory and can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.
- Molecular Weight
- 27 kDa
- Gene ID
- 7347
- NCBI Accession
- NP_005993
- Pathways
- Feeding Behaviour, Positive Regulation of fat Cell Differentiation
-