TIGIT Protein (partial) (Fc Tag)

Catalog No. ABIN5608471

Product Details

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: partial

Origin: Rabbit

Source: HEK-293 Cells

Purification tag / Conjugate: This TIGIT protein is labelled with Fc Tag.

Application: Western Blotting (WB), ELISA

Sequence: Ala 16 - Pro 142

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Endotoxin level is less than 1.0 EU per ug by the LAL method.

Biological Activity Comment:

Measured by its binding ability in a functional ELISA. Immobilized Human Nectin-2, His Tag at 5 μg/mL (100 uL/well) can bind Rabbit TIGIT, Fc Tag with a linear range of 5-78 ng/mL (QC tested).

Application Details

Application Notes: This recombinant protein can be used for E, WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM Glycine, pH 7.5

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C.

Target Details

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT (TIGIT Products)

Synonyms: VSIG9 Protein, VSTM3 Protein, WUCAM Protein, ENSMUSG00000071552 Protein, Vstm3 Protein, RGD1563191 Protein, T-cell immunoreceptor with Ig and ITIM domains Protein, T cell immunoreceptor with Ig and ITIM domains Protein, TIGIT Protein, Tigit Protein

Background: T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Molecular Weight: 40.4 kDa

Gene ID: 100343230

UniProt: G1SLC1

Pathways: Cancer Immune Checkpoints