P35 Protein
Origin: Borrelia burgdorferi
Host: Escherichia coli (E. coli)
Recombinant
ELISA, WB
1 image
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- Target
- P35
- Protein Type
- Recombinant
- Origin
- Borrelia burgdorferi
- Source
- Escherichia coli (E. coli)
- Application
- ELISA, Western Blotting (WB)
- Purification
- p35 is a fusion protein with an MBP tag and was expressed in E.coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified p35 and was estimated to be greater than 95% pure.
- Sterility
- Sterile filtered
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- Application Notes
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Application Note: p35 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 69.5 kDa for p35-MBP, (27.1 kDa for p35 and 42.4 kDa for MBP) in size corresponding to p35 by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- Expiry Date
- 6 months
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- Target
- P35
- Synonyms
- Anx1 Protein, p35 Protein, CDK5P35 Protein, CDK5R Protein, NCK5A Protein, p23 Protein, p25 Protein, p35nck5a Protein, 2010323F13Rik Protein, GFRP Protein, P35 Protein, annexin A1 Protein, cyclin dependent kinase 5 regulatory subunit 1 Protein, GTP cyclohydrolase I feedback regulator Protein, Anxa1 Protein, CDK5R1 Protein, Gchfr Protein
- Target Type
- Viral Protein
- Gene ID
- 1194146
- UniProt
- O50687
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