POLN Protein (AA 60-240) (His tag)
POLN
Origin: Hepatitis E Virus (HEV)
Host: Escherichia coli (E. coli)
Recombinant
> 90 %
SDS
1 image
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- Target See all POLN products
- POLN (Polymerase (DNA Directed) nu (POLN))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 60-240
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Origin
- Hepatitis E Virus (HEV)
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POLN protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- EVFWNHPIQR VIHNELELYC RARSGRCLEI GAHPRSINDN PNVVHRCFLR PAGRDVQRWY TAPTRGPAAN CRRSALRGLP AADRTYCFDG FSGCNFPAET GIALYSLHDM SPSDVAEAMF RHGMTRLYAA LHLPPEVLLP PGTYRTASYL LIHDGRRVVV TYEGDTSAGY NHDVSNLRSW I
- Purification
- SDS-PAGE
- Purity
- > 90 %
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Polymerase (DNA Directed) nu (POLN) (AA 1-100) protein (GST tag)
POLN Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- POLN (Polymerase (DNA Directed) nu (POLN))
- Alternative Name
- POLN (POLN Products)
- Synonyms
- POLN Protein, POL4P Protein, DNA polymerase nu Protein, polymerase (DNA directed) nu Protein, DNA polymerase N Protein, POLN Protein, Poln Protein
- Target Type
- Viral Protein
- Background
- Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m7GTP or m7GDP. GMP, GpppG, and GpppA were poor substrates for the methyltransferase. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m7GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure. Cap analogs such as m7GTP, m7GDP, et2m7GMP, and m2et7GMP inhibit the methyltransferase reaction .RNA-directed RNA polymerase plays an essential role in the virus replication. Binds to the 3'-end of the genomic RNA, probably to initiate replication ..
- Molecular Weight
- 24.5 kDa
- UniProt
- P33424
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