hCoV-OC43 Spike Protein (AA 15-344, partial) (His-SUMO Tag)
-
- Target See all hCoV-OC43 Spike (HCoV-OC43 S) products
- hCoV-OC43 Spike (HCoV-OC43 S) (Coronavirus OC43 Spike Protein (HCoV-OC43 S))
- Protein Type
- Recombinant
- Protein Characteristics
- partial, AA 15-344
-
Origin
- Human Coronavirus OC43 (HCoV-OC43)
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This hCoV-OC43 Spike protein is labelled with His-SUMO Tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- VIGDLKCTSD NINDKDTGPP PISTDTVDVT NGLGTYYVLD RVYLNTTLFL NGYYPTSGST YRNMALKGSV LLSRLWFKPP FLSDFINGIF AKVKNTKVIK DRVMYSEFPA ITIGSTFVNT SYSVVVQPRT INSTQDGDNK LQGLLEVSVC QYNMCEYPQT ICHPNLGNHR KELWHLDTGV VSCLYKRNFT YDVNADYLYF HFYQEGGTFY AYFTDTGVVT KFLFNVYLGM ALSHYYVMPL TCNSKLTLEY WVTPLTSRQY LLAFNQDGII FNAEDCMSDF MSEIKCKTQS IAPPTGVYEL NGYTVQPIAD VYRRKPNLPN CNIEAWLNDK
- Purification
- SDS-PAGE
- Purity
- > 90 %
-
Coronavirus OC43 Spike Protein (HCoV-OC43 S) protein (His tag)
HCoV-OC43 S Origin: Human Coronavirus OC43 (HCoV-OC43) Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE.
Coronavirus OC43 Spike Protein (HCoV-OC43 S) protein (His tag,ECD)HCoV-OC43 S Origin: Human Coronavirus OC43 (HCoV-OC43) Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by reducing SDS-PAGE.
Coronavirus OC43 Spike Protein (HCoV-OC43 S) (AA 15-344), (partial) protein (His tag)HCoV-OC43 S Origin: Human Coronavirus OC43 (HCoV-OC43) Host: Yeast Recombinant > 90 % SDS
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
-
- Target
- hCoV-OC43 Spike (HCoV-OC43 S) (Coronavirus OC43 Spike Protein (HCoV-OC43 S))
- Alternative Name
- OC43 Spike Glycoprotein (HCoV-OC43 S Products)
- Target Type
- Viral Protein
- Background
- S1 attaches the virion to the cell mbrane by interacting with sialic acid-containing cell receptors, initiating the infection.S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell mbrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell mbranes.
- Molecular Weight
- 53.6 kDa
- UniProt
- P36334
-
