Gag (AA 216-478) protein (GST tag)
Origin: Moloney Murine Leukemia Virus (MoMuLV)
Host: Escherichia coli (E. coli)
Recombinant
> 90 %
SDS
1 image
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- Target
- Gag
- Protein Type
- Recombinant
- Protein Characteristics
- AA 216-478
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Origin
- Moloney Murine Leukemia Virus (MoMuLV)
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- GST tag
- Application
- SDS-PAGE (SDS)
- Sequence
- PLRAGGNGQL QYWPFSSSDL YNWKNNNPSF SEDPGKLTAL IESVLITHQP TWDDCQQLLG TLLTGEEKQR VLLEARKAVR GDDGRPTQLP NEVDAAFPLE RPDWDYTTQA GRNHLVHYRQ LLLAGLQNAG RSPTNLAKVK GITQGPNESP SAFLERLKEA YRRYTPYDPE DPGQETNVSM SFIWQSAPDI GRKLERLEDL KNKTLGDLVR EAEKIFNKRE TPEEREERIR RETEEKEERR RTEDEQKEKE RDRRRHREMS KLL
- Purification
- SDS-PAGE
- Purity
- > 90 %
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- Gag
- Synonyms
- gag protein Protein, gag Protein
- Target Type
- Viral Protein
- Background
- Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release.
- Molecular Weight
- 58 kDa
- UniProt
- P03332
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