RPA2 Protein (AA 1-267, partial) (GST tag)

Catalog No. ABIN5712161

Product Details

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Protein Type: Recombinant

Protein Characteristics: AA 1-267, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RPA2 protein is labelled with GST tag.

Application: SDS-PAGE (SDS)

Sequence: MWNSGFESYG SSSYGGAGGY TQSPGGFGSP APSQAEKKSR ARAQHIVPCT ISQLLSATLV DEVFRIGNVE ISQVTIVGII RHAEKAPTNI VYKIDDMTAA PMDVRQWVDT DDTSSENTVV PPETYVKVAG HLRSFQNKKS LVAFKIMPLE DMNEFTTHIL EVINAHMVLS KANSQPSAGR APISNPGMSE AGNFGGNSFM PANGLTVAQN QVLNLIKACP RPEGLNFQDL KNQLKHMSVS SIKQAVDFLS NEGHIYSTVD DDHFKST

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RFA2 (RPA2 Products)

Synonyms: REPA2 Protein, RPA32 Protein, 153194_at Protein, CG9273 Protein, Dmel\\CG9273 Protein, dRPA2 Protein, rpa Protein, zgc:109822 Protein, ik:tdsubc_2g1 Protein, xx:tdsubc_2g1 Protein, repa2 Protein, rpa32 Protein, RPA2 Protein, GB13976 Protein, LOC100230445 Protein, AA409079 Protein, AI325195 Protein, AU020965 Protein, RPA34 Protein, Rf-A2 Protein, replication protein A2 Protein, Replication protein A2 Protein, replication protein A2, 32kDa Protein, replication protein A 32 kDa subunit Protein, RPA2 Protein, Rpa2 Protein, rpa2 Protein, LOC725418 Protein, RPA2_2 Protein

Background: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance

Molecular Weight: 56.3 kDa

UniProt: P15927

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA