TPST1 Protein (AA 26-370) (His tag)
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- Target See all TPST1 Proteins
- TPST1 (Tyrosylprotein Sulfotransferase 1 (TPST1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-370
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This TPST1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADPQHAMECH HRIEERSQPV KLESTRTTVR TGLDLKANKT FAYHKDMPLI FIGGVPRSGT TLMRAMLDAH PDIRCGEETR VIPRILALKQ MWSRSSKEKI RLDEAGVTDE VLDSAMQAFL LEIIVKHGEP APYLCNKDPF ALKSLTYLSR LFPNAKFLLM VRDGRASVHS MISRKVTIAG FDLNSYRDCL TKWNRAIETM YNQCMEVGYK KCMLVHYEQL VLHPERWMRT LLKFLQIPWN HSVLHHEEMI GKAGGVSLSK VERSTDQVIK PVNVGALSKW VGKIPPDVLQ DMAVIAPMLA KLGYDPYANP PNYGKPDPKI IENTRRVYKG EFQLPDFLKE KPQTEQVEHH HHHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product TPST1 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- TPST1 (Tyrosylprotein Sulfotransferase 1 (TPST1))
- Alternative Name
- TPST1 (TPST1 Products)
- Synonyms
- MGC82552 Protein, MGC97723 Protein, R75054 Protein, Tango13a Protein, TANGO13A Protein, fj54g11 Protein, wu:fj54g11 Protein, tyrosylprotein sulfotransferase 1 Protein, tyrosylprotein sulfotransferase 1 L homeolog Protein, protein-tyrosine sulfotransferase 1 Protein, TPST1 Protein, tpst1.L Protein, tpst1 Protein, Tpst1 Protein
- Background
- TPST1, also known as protein-tyrosine sulfotransferase 1, is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. It utilizes 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfonate donor and binds proteins with target tyrosine residues to eventually form the tyrosine O-sulfate ester group and the desulfonated 3'-phosphoadenosine-5'-phosphate (PAP). Recombinant human TPST1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 40.6kDa (354aa) 40-57KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_003587
- UniProt
- O60507
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